Overview

  • Product name
  • Description
    Rabbit polyclonal to ADCY8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ADCY8 aa 952-982 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P40145

  • Positive control
    • HL60 cell lysate; Human brain tissue; HL60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 140 kDa.

Target

Images

  • Anti-ADCY8 antibody (ab175874) at 1/1000 dilution + HL60 cell lysate at 35 µg

    Predicted band size: 140 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue labeling ADCY8 using ab175874 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of HL-60 cells labeling ADCY8 using ab175874 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175874 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175874.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up