Overview

  • Product name

    Anti-ADCY9/AC9 antibody [EPR16188]
    See all ADCY9/AC9 primary antibodies
  • Description

    Rabbit monoclonal [EPR16188] to ADCY9/AC9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ADCY9/AC9 aa 550-800. The exact sequence is proprietary.
    Database link: O60503

  • Positive control

    • A549, K562, Mouse heart, Mouse spleen, Rat brain, PC12 and NIH 3T3 lysates.
  • General notes

     

     

     This product was previously labelled as ADCY9

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 151 kDa (predicted molecular weight: 151 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      May play a fundamental role in situations where fine interplay between intracellular calcium and cAMP determines the cellular function. May be a physiologically relevant docking site for calcineurin.
    • Tissue specificity

      Expressed in multiple cells of the lung, with expression highest in airway smooth muscle.
    • Sequence similarities

      Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
      Contains 2 guanylate cyclase domains.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADCY 9 antibody
      • ADCY9 antibody
      • ADCY9_HUMAN antibody
      • Adenylate cyclase 9 antibody
      • Adenylate cyclase type 9 antibody
      • Adenylate cyclase type IX antibody
      • Adenylyl cyclase 9 antibody
      • ATP pyrophosphate lyase 9 antibody
      • ATP pyrophosphate-lyase 9 antibody
      • KIAA0520 antibody
      • Type IX adenylyl cyclase antibody
      • Type IX ATP pyrophosphate lyase antibody
      see all

    Images

    • All lanes : Anti-ADCY9/AC9 antibody [EPR16188] (ab191423) at 1/10000 dilution

      Lane 1 : A549 lysate
      Lane 2 : K562 lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 151 kDa

    • All lanes : Anti-ADCY9/AC9 antibody [EPR16188] (ab191423) at 1/2000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse spleen lysate
      Lane 3 : Rat brain lysate
      Lane 4 : PC12 lysate
      Lane 5 : NIH 3T3 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 151 kDa

    References

    This product has been referenced in:

    • Wang T  et al. Sorafenib promotes sensory conduction function recovery via miR-142-3p/AC9/cAMP axis post dorsal column injury. Neuropharmacology 148:347-357 (2019). Read more (PubMed: 30710569) »
    • Tan X  et al. The epithelial-to-mesenchymal transition activator ZEB1 initiates a prometastatic competing endogenous RNA network. J Clin Invest 128:1267-1282 (2018). Read more (PubMed: 29324442) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    HEK cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jun 24 2019

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    brain
    Blocking step
    5% normal donkey serum, 0.1 TritinX-100, 2% BSA in 0.1M PB 7.4 as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 22°C
    Fixative
    Paraformaldehyde

    Dr. Jana Aradska

    Verified customer

    Submitted Nov 20 2015

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