Recombinant Anti-ADCY9/AC9 antibody [EPR16188] (ab191423)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16188] to ADCY9/AC9
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ADCY9/AC9 antibody [EPR16188]
See all ADCY9/AC9 primary antibodies -
Description
Rabbit monoclonal [EPR16188] to ADCY9/AC9 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549, K562, Mouse heart, Mouse spleen, Rat brain, PC12 and NIH 3T3 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16188 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 151 kDa (predicted molecular weight: 151 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 151 kDa (predicted molecular weight: 151 kDa). |
Target
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Function
May play a fundamental role in situations where fine interplay between intracellular calcium and cAMP determines the cellular function. May be a physiologically relevant docking site for calcineurin. -
Tissue specificity
Expressed in multiple cells of the lung, with expression highest in airway smooth muscle. -
Sequence similarities
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 2 guanylate cyclase domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 115 Human
- Entrez Gene: 11515 Mouse
- Entrez Gene: 302950 Rat
- Omim: 603302 Human
- SwissProt: O60503 Human
- SwissProt: P51830 Mouse
- Unigene: 391860 Human
- Unigene: 610484 Human
see all -
Alternative names
- ADCY 9 antibody
- ADCY9 antibody
- ADCY9_HUMAN antibody
see all
Images
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All lanes : Anti-ADCY9/AC9 antibody [EPR16188] (ab191423) at 1/10000 dilution
Lane 1 : A549 lysate
Lane 2 : K562 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 151 kDa -
All lanes : Anti-ADCY9/AC9 antibody [EPR16188] (ab191423) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : PC12 lysate
Lane 5 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 151 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (9)
ab191423 has been referenced in 9 publications.
- Liu J et al. miR-143-null Is against Diet-Induced Obesity by Promoting BAT Thermogenesis and Inhibiting WAT Adipogenesis. Int J Mol Sci 23:N/A (2022). PubMed: 36361843
- Liu J et al. LPS-Induced Inhibition of miR-143 Expression in Brown Adipocytes Promotes Thermogenesis and Fever. Int J Mol Sci 23:N/A (2022). PubMed: 36430282
- Li X et al. miR-142-3p targets AC9 to regulate sciatic nerve injury-induced neuropathic pain by regulating the cAMP/AMPK signalling pathway. Int J Mol Med 47:561-572 (2021). PubMed: 33416140
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
- Hampson E et al. P-Rex1 Controls Sphingosine 1-Phosphate Receptor Signalling, Morphology, and Cell-Cycle Progression in Neuronal Cells. Cells 10:N/A (2021). PubMed: 34572121
- Cortes-Troncoso J et al. T cell exosome-derived miR-142-3p impairs glandular cell function in Sjögren's syndrome. JCI Insight 5:N/A (2020). PubMed: 32376798
- Simard M et al. A Tissue-Engineered Human Psoriatic Skin Model to Investigate the Implication of cAMP in Psoriasis: Differential Impacts of Cholera Toxin and Isoproterenol on cAMP Levels of the Epidermis. Int J Mol Sci 21:N/A (2020). PubMed: 32717879
- Wang T et al. Sorafenib promotes sensory conduction function recovery via miR-142-3p/AC9/cAMP axis post dorsal column injury. Neuropharmacology 148:347-357 (2019). PubMed: 30710569
- Tan X et al. The epithelial-to-mesenchymal transition activator ZEB1 initiates a prometastatic competing endogenous RNA network. J Clin Invest 128:1267-1282 (2018). PubMed: 29324442