Product nameAnti-Adducin antibody
DescriptionRabbit polyclonal to Adducin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 81 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceAdducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Cellular localizationCytoplasm › cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
- ADD1 antibody
- ADD2 antibody
- ADD3 antibody
All lanes : Anti-Adducin antibody (ab176699) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 30 seconds
Detection of Adducin by Western Blot of Immunprecipitate.
Lane 1: ab176699 at 1 µg/ml staining Adducin in 293T whole cell lysate immunoprecipitated using ab176699 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
ab176699 has not yet been referenced specifically in any publications.