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Previously labelled as Adenine Nucleotide Translocase 1.
Our Abpromise guarantee covers the use of ab102032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Adenine Nucleotide Translocase 1 / ANT 1 with ab102032 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes and their processes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Bottom left - DAPI. Top right - Adenine Nucleotide Translocase 1. Top left - Merge.
ab102032 staining Adenine Nucleotide Translocase 1 / ANT 1 from HEK293 cell line from Human by ICC/IF (Immunocytochemistry/immunofluorescence).Cells were fixed with paraformaldehyde,permeabilized with 1% Triton X-100.Samples were incubated with primary antibody for 16 hours at 4 °C.A diluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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