Key features and details
- Rabbit polyclonal to Adenine Nucleotide Translocase 1/ANT 1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Adenine Nucleotide Translocase 1/ANT 1 antibody
See all Adenine Nucleotide Translocase 1/ANT 1 primary antibodies
DescriptionRabbit polyclonal to Adenine Nucleotide Translocase 1/ANT 1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- RT-4 and U-251 MG cell lysates; PC-3 cells.
Previously labelled as Adenine Nucleotide Translocase 1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa.|
FunctionCatalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
Involvement in diseaseDefects in SLC25A4 are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 2 (PEOA2) [MIM:609283]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
Sequence similaritiesBelongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- AAC1 antibody
- Adenine Nucleotide Translocase 1 antibody
- Adenine nucleotide translocator 1 (skeletal muscle) antibody
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized PC-3 cells labeling Adenine Nucleotide Translocator 2 with ab220408 at 4 μg/ml (green).
All lanes : Anti-Adenine Nucleotide Translocase 1/ANT 1 antibody (ab220408) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Predicted band size: 33 kDa
Anti-Adenine Nucleotide Translocase 1/ANT 1 antibody (ab220408) at 0.4 µg + Testis tissue lysate
Predicted band size: 33 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab220408 has not yet been referenced specifically in any publications.