Overview

  • Product name

    Anti-Adenine Nucleotide Translocase 1/ANT 1 antibody
    See all Adenine Nucleotide Translocase 1/ANT 1 primary antibodies
  • Description

    Rabbit polyclonal to Adenine Nucleotide Translocase 1/ANT 1
  • Host species

    Rabbit
  • Specificity

    This antibody is named based on homology. It can also recognise ADP/ATP translocase 2, SLC25A31, SLC25A5 and SLC25A6 due to homology of 93% in immunogen sequence.

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Adenine Nucleotide Translocase 1/ANT 1 aa 19-46.
    Sequence:

    AAVSKTAVAPIERVKLLLQVQHASKQIS


    Database link: P12235

  • Positive control

    • IHC-P: Human heart muscle tissue. WB: RT-4 and U-251 MG cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab221956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 33 kDa.

Target

  • Function

    Catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
  • Involvement in disease

    Defects in SLC25A4 are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 2 (PEOA2) [MIM:609283]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAC1 antibody
    • Adenine Nucleotide Translocase 1 antibody
    • Adenine nucleotide translocator 1 (skeletal muscle) antibody
    • Adenine nucleotide translocator 1 antibody
    • ADP antibody
    • ADP ATP carrier protein 1 antibody
    • ADP ATP carrier protein heart/skeletal muscle isoform T1 antibody
    • ADP/ATP translocase 1 antibody
    • ADT1_HUMAN antibody
    • ANT 1 antibody
    • ANT antibody
    • ANT1 antibody
    • ATP carrier protein 1 antibody
    • ATP carrier protein antibody
    • heart/skeletal muscle isoform T1 antibody
    • PEO2 antibody
    • PEO3 antibody
    • SLC25A4 antibody
    • Solute carrier family 25 member 4 antibody
    • T1 antibody
    see all

Images

  • All lanes : Anti-Adenine Nucleotide Translocase 1/ANT 1 antibody (ab221956) at 1/100 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate

    Predicted band size: 33 kDa

  • Paraffin embedded human heart muscle tissue stained for Adenine Nucleotide Translocase 1/ANT 1 with ab221956 (1/20 dilution) in immunohistochemical analysis.

References

ab221956 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Renal proximal tubular cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Renal proximal tubular cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Liad Hinden

Verified customer

Submitted Oct 22 2018

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