Overview

  • Product name

    Adenine Nucleotide Translocase (ANT) Immunocapture Kit
  • Assay type

    Quantitative
  • Species reactivity

    Reacts with: Cow, Human
  • Product overview

    Capture monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture Adenine Nucleotide Translocase from heart mitochondria.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

Properties

Images

  • Adenine Nucleotide Translocase immunoprecipitation using antibody ab109864/MS101c crosslinked to protein G-agarose beads as product ab109713

Protocols

References

This product has been referenced in:

  • Duarte FV  et al. Dibenzofuran-induced mitochondrial dysfunction: Interaction with ANT carrier. Toxicol In Vitro 27:2160-2168 (2013). Read more (PubMed: 24008156) »
  • Mailloux RJ  et al. Hexokinase II acts through UCP3 to suppress mitochondrial reactive oxygen species production and maintain aerobic respiration. Biochem J 437:301-11 (2011). Read more (PubMed: 21554247) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Frozen mitochondria should not be a problem for any immunoprecipitation kits.

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Answer

Thank you for your reply.

The kit contains 25 uL (settled volume) of beads conjugated to 250 ug of antibody. This is enough to run 5 tests ifyouuse5 uL beads for each test. The 5 uL recommended minimum is for any sample, including mitochondria. We recommend to use 5 uLof beads as the minimum amount, and you can try to use less amount of beads, but according to our experience, it is difficult to handle and easy to lose the beads during the procedure.But please keep in mind thatthe volume we talk about is the solid beads volume. It is not the volume of the slurry.

I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for contacting us.

Yes, the Mitochondria Isolation Kit for Tissue (ab110168) is an ideal kit to use to isolate mitochondria for use with the ANT Immunocapture Kit (ab109713).

For the ANT protocol, "The smallest working amount of beads is around 5 μl of solid beads in a small microtube (e.g. a 500 μl tube). It is not practical to use a volume smaller than this for immunocapture procedure, therefore it is recommended that when wishing to use less beads researchers dilute the concentrated Abcam’s beads with unloaded agarose beads, giving a workable volume of beads. The yield from such diluted beads will of course be less. Plain beads for dilution are available from Abcam."

https://www.abcam.com/ps/products/109/ab109713/documents/ab109713%20Adenine%20Nucleotide%20Translocase%20Human%20and%20Bovine%20Immunocapture%20Kit%20v2%20(Website).pdf

For muscle mitochondria, the minimal starting about is 200 ug and the recommended starting amount is 2-5 mg.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

ab109864 anti-ANT beads uses the anti-ANT monoclonal antibody ab110322. This antibody is specific to Mouse, Rat, Cow, Human, Caenorhabditis elegans ANT.

The antibody was raised against full length recombinant human ANT, but the exact binding site is unknown.

I blasted human and mouse and there is a 95% identity - so that is excellent similarity.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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