Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)


  • Product name
    Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7]
  • Description
    Mouse monoclonal [5F51BB5AG7] to Adenine Nucleotide Translocase Immunocapture
  • Host species
  • Tested applications
    Suitable for: IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Recombinant Human Adenine Nucleotide Translocase

  • Positive control
    • Bovine and Human heart mitochondria This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab109864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 100 µg/mg of lysate.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Relevance
    ANT is a reversible transporter of ATP and ADP. During oxidative phosphorylation (OXPHOS) the protein exchanges ATP out for ADP in, however, in the absence of OXPHOS ANT works in reverse to maintain mitochondrial membrane potential. A subset of patients with heart related diseases have been shown to harbor mutations in the ANT gene or to develop an autoimmune reaction against the protein. There are a number of proteins found in mitochondria that, under circumstances favoring apoptosis, coalesce to form the so-called permeability transition pore. The proteins identified as a part of the PTP include cytosolic hexokinase, outer membrane porin (also called the voltage dependent anion channel or VDAC), adenylate cyclase in the intermembrane space, the adenine nucleotide transporter (ANT) and the peripheral benzodiazepine receptor protein of the inner membrane along with cyclophilin D of the matrix space. Hexokinase, porin and ANT all occur as isoforms and it remains to be determined whether there is specificity of these different forms for the PTP.
  • Cellular localization


  • IHC image of Adenine Nucleotide Translocase staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109864, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocapture of Adenine Nucleotide Translocase from Bovine heart mitochondria using ab109864 at a concentration of 100 µg/mg lysate.


This product has been referenced in:
  • Mailloux RJ  et al. Hexokinase II acts through UCP3 to suppress mitochondrial reactive oxygen species production and maintain aerobic respiration. Biochem J 437:301-11 (2011). Read more (PubMed: 21554247) »
  • Shulga N  et al. Sirtuin-3 deacetylation of cyclophilin D induces dissociation of hexokinase II from the mitochondria. J Cell Sci 123:894-902 (2010). Read more (PubMed: 20159966) »

See all 4 Publications for this product

Customer reviews and Q&As

I am very pleased to hear you would like to accept our offer and test ab110322 and ab109864 in IP on mouse tissue. This code will give you 1 free PRIMARY ANTIBODY for each code before the expiration date. To redeem this offer, please submit an Abreview...

Read More

Thank you for contacting us.

We unfortunately at this time Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322) has not been tested for use in IP. However, this does not mean that it will not work, just that we have not t...

Read More

Thanks for your phone call last week and I apologize for the extended delay getting back to you. There is an error on the datasheet, and the recommended antibody ratio of 100 ug per mg of sample is actually for a different Mitosciences product in which...

Read More


Sign up