Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
Key features and details
- Rabbit polyclonal to Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody
See all Adenine Nucleotide Translocator 2/ANT 2+Adenine Nucleotide Translocase 1/ANT 1 primary antibodies -
Description
Rabbit polyclonal to Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 -
Host species
Rabbit -
Specificity
This antibody can also recognise ADP/ATP translocase 2, SLC25A31, SLC25A5 and SLC25A6 due to homology of 93% in immunogen sequence.
-
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
-
Immunogen
Recombinant fragment aa 1-100.
Database link: P12235 -
Positive control
- IHC-P: Human cerebellum, kidney and duodenum tissue. WB: RT-4 and U-251 MG cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa.
|
| Notes |
|---|
|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa. |
Target
-
Cellular localization
Adenine Nucleotide Translocator 2/ANT 2: Mitochondrion inner membrane. Adenine Nucleotide Translocase 1/ANT 1: Mitochondrion inner membrane. -
Database links
- Entrez Gene: 282478 Cow
- Entrez Gene: 282479 Cow
- Entrez Gene: 291 Human
- Entrez Gene: 292 Human
- Entrez Gene: 11739 Mouse
- Entrez Gene: 11740 Mouse
- Entrez Gene: 25176 Rat
- Entrez Gene: 85333 Rat
see all -
Alternative names
- 2F1 antibody
- AAC1 antibody
- AAC2 antibody
see all
Images
-
Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)All lanes : Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956) at 0.4 µg/ml
Lane 1 : SLC25A4 (Human) Recombinant Protein (ANT1) cell lysate at 0.1 µg/ml
Lane 2 : Recombinant Human Adenine Nucleotide Translocator 2/ANT 2 protein (ab157891) cell lysate at 0.1 µg/ml
Lane 3 : Human Skeletal Muscle cell lysate at 20 µg
Lane 4 : Human Liver cell lysate at 20 µg
Lane 5 : Mouse Skeletal Muscle cell lysate at 20 µg
Lane 6 : Mouse Liver cell lysate at 20 µg
Lane 7 : Rat Skeletal Muscle cell lysate at 20 µg
Lane 8 : Rat Liver cell lysate at 20 µg
Predicted band size: 33 kDa
Observed band size: 33 kDaFalse colour image of Western blot: Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody staining at 0.4 µg/ml, shown in green; loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, ab221956 was shown to bind specifically to Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)Paraffin embedded human cerebellum tissue stained for Adenine Nucleotide Translocase 1/ANT 1 with ab221956 (1/20 dilution) in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)Paraffin embedded human kidney tissue stained for Adenine Nucleotide Translocase 1/ANT 1 with ab221956 (1/20 dilution) in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)Paraffin embedded human duodenum tissue stained for Adenine Nucleotide Translocase 1/ANT 1 with ab221956 (1/20 dilution) in immunohistochemical analysis.
-
Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)All lanes : Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Predicted band size: 33 kDa
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab221956 has not yet been referenced specifically in any publications.
