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    adenine-nucleotide-translocator-1--2ant-1--2-antibody-ab221956.pdf

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Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)

  • Datasheet
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Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
  • Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)

Key features and details

  • Rabbit polyclonal to Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Recombinant Human Adenine Nucleotide Translocator 2/ANT 2 protein (ab157891)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody
    See all Adenine Nucleotide Translocator 2/ANT 2+Adenine Nucleotide Translocase 1/ANT 1 primary antibodies
  • Description

    Rabbit polyclonal to Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2
  • Host species

    Rabbit
  • Specificity

    This antibody can also recognise ADP/ATP translocase 2, SLC25A31, SLC25A5 and SLC25A6 due to homology of 93% in immunogen sequence.

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment aa 1-100.
    Database link: P12235

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human cerebellum, kidney and duodenum tissue. WB: RT-4 and U-251 MG cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Adenine Nucleotide Translocator 2/ANT 2 protein (ab157891)
  • Related Products

    • Recombinant Human Adenine Nucleotide Translocator 2/ANT 2 protein (ab157891)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab221956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (1)
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa.
Notes
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa.

Target

  • Cellular localization

    Adenine Nucleotide Translocator 2/ANT 2: Mitochondrion inner membrane. Adenine Nucleotide Translocase 1/ANT 1: Mitochondrion inner membrane.
  • Database links

    • Entrez Gene: 282478 Cow
    • Entrez Gene: 282479 Cow
    • Entrez Gene: 291 Human
    • Entrez Gene: 292 Human
    • Entrez Gene: 11739 Mouse
    • Entrez Gene: 11740 Mouse
    • Entrez Gene: 25176 Rat
    • Entrez Gene: 85333 Rat
    • Omim: 103220 Human
    • Omim: 300150 Human
    • SwissProt: P02722 Cow
    • SwissProt: Q8SQH5 Cow
    • SwissProt: P05141 Human
    • SwissProt: P12235 Human
    • SwissProt: P48962 Mouse
    • SwissProt: P51881 Mouse
    • SwissProt: O46373 Rabbit
    • SwissProt: Q05962 Rat
    • SwissProt: Q09073 Rat
    • Unigene: 246506 Human
    • Unigene: 632282 Human
    • Unigene: 16228 Mouse
    • Unigene: 371544 Mouse
    • Unigene: 102263 Rat
    • Unigene: 4092 Rat
    see all
  • Alternative names

    • 2F1 antibody
    • AAC1 antibody
    • AAC2 antibody
    • ADP ATP carrier protein 1 antibody
    • ADP ATP carrier protein 2 antibody
    • ANT1 antibody
    • ANT2 antibody
    • OTTHUMP00000024324 antibody
    • PEO2 antibody
    • PEO3 antibody
    • T1 antibody
    • T2 antibody
    • T3 antibody
    see all

Images

  • Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
    Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
    All lanes : Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956) at 0.4 µg/ml

    Lane 1 : SLC25A4 (Human) Recombinant Protein (ANT1) cell lysate at 0.1 µg/ml
    Lane 2 : Recombinant Human Adenine Nucleotide Translocator 2/ANT 2 protein (ab157891) cell lysate at 0.1 µg/ml
    Lane 3 : Human Skeletal Muscle cell lysate at 20 µg
    Lane 4 : Human Liver cell lysate at 20 µg
    Lane 5 : Mouse Skeletal Muscle cell lysate at 20 µg
    Lane 6 : Mouse Liver cell lysate at 20 µg
    Lane 7 : Rat Skeletal Muscle cell lysate at 20 µg
    Lane 8 : Rat Liver cell lysate at 20 µg

    Predicted band size: 33 kDa
    Observed band size: 33 kDa



    False colour image of Western blot: Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody staining at 0.4 µg/ml, shown in green; loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, ab221956 was shown to bind specifically to Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)

    Paraffin embedded human cerebellum tissue stained for Adenine Nucleotide Translocase 1/ANT 1 with ab221956 (1/20 dilution) in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)

    Paraffin embedded human kidney tissue stained for Adenine Nucleotide Translocase 1/ANT 1 with ab221956 (1/20 dilution) in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)

    Paraffin embedded human duodenum tissue stained for Adenine Nucleotide Translocase 1/ANT 1 with ab221956 (1/20 dilution) in immunohistochemical analysis.

  • Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
    Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956)
    All lanes : Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab221956) at 1/100 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate

    Predicted band size: 33 kDa

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab221956? Please let us know so that we can cite the reference in this datasheet.

ab221956 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Western blot abreview for Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Renal proximal tubular cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Renal proximal tubular cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

DR. Liad Hinden

Verified customer

Submitted Oct 22 2018

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