Overview

  • Product name

    Anti-Adenine Nucleotide Translocator 2/ANT 2 antibody
    See all Adenine Nucleotide Translocator 2/ANT 2 primary antibodies
  • Description

    Rabbit polyclonal to Adenine Nucleotide Translocator 2/ANT 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Adenine Nucleotide Translocator 2/ANT 2 aa 19-46.
    Sequence:

    AAVSKTAVAPIERVKLLLQVQHASKQIS


    Database link: P05141

  • Positive control

    • IHC-P: Human heart muscle tissue. WB: RT-4 and U-251 MG cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab221956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 33 kDa.

Target

  • Function

    Catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2F1 antibody
    • AAC2 antibody
    • Adenine nucleotide translocator 2 antibody
    • ADP antibody
    • ADP ATP carrier protein 2 antibody
    • ADP/ATP translocase 2 antibody
    • ADT2_HUMAN antibody
    • ANT 2 antibody
    • ANT2 antibody
    • ATP carrier protein 2 antibody
    • ATP carrier protein antibody
    • fibroblast isoform antibody
    • OTTHUMP00000024324 antibody
    • SLC25A5 antibody
    • Solute carrier family 25 (mitochondrial carrier adenine nucleotide translocator) member 5 antibody
    • Solute carrier family 25 member 5 antibody
    • T2 antibody
    • T3 antibody
    see all

Images

  • All lanes : Anti-Adenine Nucleotide Translocator 2/ANT 2 antibody (ab221956) at 1/100 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate

    Predicted band size: 33 kDa

  • Paraffin embedded human heart muscle tissue stained for Adenine Nucleotide Translocator 2/ANT 2 with ab221956 (1/20 dilution) in immunohistochemical analysis.

References

ab221956 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Renal proximal tubular cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Renal proximal tubular cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Liad Hinden

Verified customer

Submitted Oct 22 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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