Anti-APC2 antibody (ab106600)
Key features and details
- Rabbit polyclonal to APC2
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-APC2 antibody
See all APC2 primary antibodies -
Description
Rabbit polyclonal to APC2 -
Host species
Rabbit -
Specificity
ab106600 is predicted to not cross-react with other Apc family members. At least four isoforms of Apc2 are known to exist. -
Tested applications
Suitable for: ICC/IF, WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human APC2 (internal sequence).
Database link: O95996 -
Positive control
- HeLa cells and cell lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 20 µg/ml.
|
|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 244 kDa.
|
|
ICC |
Use a concentration of 5 µg/ml.
|
Notes |
---|
ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 244 kDa. |
ICC
Use a concentration of 5 µg/ml. |
Target
-
Function
Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling. -
Tissue specificity
Widely expressed (at protein level). Specifically expressed in the CNS. -
Sequence similarities
Belongs to the adenomatous polyposis coli (APC) family.
Contains 6 ARM repeats. -
Developmental stage
Expressed in fetal brain. -
Cellular localization
Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasm. Cytoplasm, perinuclear region. Associated with actin filaments (PubMed:11691822). Associated with microtubule network (PubMed:10644998, PubMed:11691822). - Information by UniProt
-
Database links
- Entrez Gene: 10297 Human
- SwissProt: O95996 Human
- Unigene: 446376 Human
-
Alternative names
- Adenomatous polyposis coli protein 2 antibody
- Adenomatous polyposis coli protein-like antibody
- APC-like antibody
see all
Images
-
Lane 1 : Anti-APC2 antibody (ab106600) at 1 µg/ml
Lane 2 : Anti-APC2 antibody (ab106600) at 2 µg/ml
All lanes : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 244 kDa -
Immunofluorescence of APC2 in HeLa cells with ab106600 at 20 ug/mL.
-
Immunocytochemical analysis of ab106600 at 5 µg/ml in HeLa cells.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab106600 has not yet been referenced specifically in any publications.