Product nameAnti-Adenosine A1 Receptor antibody
See all Adenosine A1 Receptor primary antibodies
DescriptionRabbit polyclonal to Adenosine A1 Receptor
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Adenosine A1 Receptor (C terminal).
Database link: P30542
- WB: HeLa and HEK-293Trap cell lysates. ICC/IF: SK-N-BE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 36.5 kDa (predicted molecular weight: 13,36.5 kDa).|
FunctionReceptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- A1AR antibody
- A1R antibody
- AA1R antibody
4% formaldehyde-fixed SK-N-BE cells stained for Adenosine A1 Receptor (green) using ab223377 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223377 (D) Merge.
All lanes : Anti-Adenosine A1 Receptor antibody (ab223377) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293Trap cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 13,36.5 kDa
Observed band size: 36.5 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
Blocking buffer: 5% skimmed milk in TBST.
Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.
ab223377 has not yet been referenced specifically in any publications.