Anti-Adenosine A2b Receptor/ADORA2B antibody (ab229671)
Key features and details
- Rabbit polyclonal to Adenosine A2b Receptor/ADORA2B
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Adenosine A2b Receptor/ADORA2B antibody -
Description
Rabbit polyclonal to Adenosine A2b Receptor/ADORA2B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Adenosine A2b Receptor/ADORA2B (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P29275 -
Positive control
- WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. Mouse and rat brain tissue lysates. IHC-P: Mouse and rat brain tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/1000.
|
|
WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
|
Notes |
---|
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
Target
-
Function
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 136 Human
- Entrez Gene: 11541 Mouse
- Entrez Gene: 29316 Rat
- Omim: 600446 Human
- SwissProt: P29275 Human
- SwissProt: Q60614 Mouse
- SwissProt: P29276 Rat
- Unigene: 167046 Human
see all -
Alternative names
- AA2BR_HUMAN antibody
- Adenosine receptor A2b antibody
- adora 2 antibody
see all
Images
-
All lanes : Anti-Adenosine A2b Receptor/ADORA2B antibody (ab229671) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa10% SDS-PAGE gel.
-
Paraffin-embedded mouse brain tissue stained for Adenosine A2b Receptor/ADORA2B using ab229671 at 1/500 dilution in immunohistochemical analysis.
-
All lanes : Anti-Adenosine A2b Receptor/ADORA2B antibody (ab229671) at 1/3000 dilution
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 36 kDa -
Paraffin-embedded rat brain tissue stained for Adenosine A2b Receptor/ADORA2B using ab229671 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab229671 has been referenced in 1 publication.
- Jin H et al. Increased Extracellular Adenosine in Radiotherapy-Resistant Breast Cancer Cells Enhances Tumor Progression through A2AR-Akt-ß-Catenin Signaling. Cancers (Basel) 13:N/A (2021). PubMed: 33925516