Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab40002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • Cellular localization
    Multi-pass membrane protein.
  • Database links
  • Alternative names
    • Adenosine receptor A2b antibody
    • adora 2 antibody
    • adora 2b antibody
    • ADORA2 antibody
    • ADORA2B antibody
    • HGNC:264 antibody
    • Netrin 1 receptor antibody
    see all

Images

  • ab40002 (2µg/ml) staining of paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules  following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.

  • Anti-Adenosine A2b Receptor antibody (ab40002) at 0.5 µg/ml + 100ug of rat kidney cortex lysate.

    Secondary
    Bovine-anti goat IgG conjugated to HRP at 1/7500 dilution

    Performed under reducing conditions.

    Predicted band size: 37 kDa

    See Abreview

  • ab40002 at 3ug/ml staining Adenosine A2b receptor in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9.0). The HRP staining procedure was used for detection.

References

This product has been referenced in:
  • Zhou Y  et al. The adenosine A2b receptor promotes tumor progression of bladder urothelial carcinoma by enhancing MAPK signaling pathway. Oncotarget 8:48755-48768 (2017). Read more (PubMed: 28548944) »
  • Iriyama T  et al. Elevated placental adenosine signaling contributes to the pathogenesis of preeclampsia. Circulation 131:730-41 (2015). Read more (PubMed: 25538227) »
See all 5 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
Sample
Human Tissue sections (kidney)
Specification
kidney
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Sep 06 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Rabbit: aorta, liver, heart)
Loading amount
20 µg
Specification
Rabbit: aorta, liver, heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Kamil Witkowski

Verified customer

Submitted Apr 09 2013

Question
Answer

Thank you for contacting us.

The immunogen sequence homology is 90% with rabbit sequence. This antibody most likely will work.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

DISCOUNT CODE: ***
Expiration date: December 9, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab40002 in ICC/IFand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

DISCOUNT CODE: ************ - For ab4002
Expiration date: **********

I am very pleased to hear you would like to accept our offer and test ab40002 in rhesus macaque. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for rhesus macaque and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

I now have some additional information to share with you in regards to the antibodies I discussed in my last email against adenosine receptors.

Anti-Adenosine Receptor A2a

Unfortunately I can confirm that the other two antibodies I suggested against Adenosine Receptor A2a (ab97583 and ab106143) may not be suitable for what you are trying to do. Ab106143 binds in the cytoplasmic region whist the immunogen used to raise ab97583 (residues 255-305) spans the transmembrane, extracellular and cytoplasmic region. It is therefore difficult to tell if ab97583 recognises the extracellular domain but as it is only 8 residues of the whole 50 residue portion, is it unlikely.

Anti-Adenosine Receptor A3

The immunogen used to raisethe ab104502 antibody was taken from the cytoplasmic region of the protein.

I am sorry that I could not bring better news. It seems that the only antibody we can offer which definitely recognises the extracellular region and has been used in flow cytometry previously isagainst the A1 receptor (https://www.abcam.com/Adenosine-A1-Receptor-antibody-EPR6179-ab124780.html).

IF Ican be of any more help please do let me know. I wish you all the best with your research.

Read More
Application
Western blot
Sample
Rat Tissue lysate - whole (Kidney Cortex)
Loading amount
100 µg
Specification
Kidney Cortex
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Dr. prit gill

Verified customer

Submitted Apr 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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