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Cardiovascular Hypoxia Hypoxia-Regulated
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Anti-Adenosine A2b Receptor/ADORA2B antibody (ab135865)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Western blot - Anti-Adenosine A2b Receptor/ADORA2B antibody (ab135865)

    Key features and details

    • Rabbit polyclonal to Adenosine A2b Receptor/ADORA2B
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Adenosine A2b Receptor/ADORA2B antibody
      See all Adenosine A2b Receptor/ADORA2B primary antibodies
    • Description

      Rabbit polyclonal to Adenosine A2b Receptor/ADORA2B
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to Human Adenosine A2b Receptor/ADORA2B (C terminal).

    • Positive control

      • HT29 cell lysates.
    • General notes

       This product was previously labelled as Adenosine A2b Receptor

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20ºC.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

      PBS is without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab135865 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Hypoxia
      • Hypoxia-Regulated
      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Epigenetics and Nuclear Signaling
      • Cardiovascular/Immune
      • Hypoxia
      • Hypoxia regulated
      • Cancer
      • Invasion/microenvironment
      • Hypoxia
      • Hypoxia-related proteins
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab135865 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 37 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 37 kDa.

    Target

    • Relevance

      Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
    • Cellular localization

      Multi-pass membrane protein.
    • Database links

      • Entrez Gene: 136 Human
      • Omim: 600446 Human
      • SwissProt: P29275 Human
      • Unigene: 167046 Human
      • Alternative names

        • Adenosine receptor A2b antibody
        • adora 2 antibody
        • adora 2b antibody
        • ADORA2 antibody
        • ADORA2B antibody
        • HGNC:264 antibody
        • Netrin 1 receptor antibody
        see all

      Images

      • Western blot - Anti-Adenosine A2b Receptor/ADORA2B antibody (ab135865)
        Western blot - Anti-Adenosine A2b Receptor/ADORA2B antibody (ab135865)
        Anti-Adenosine A2b Receptor/ADORA2B antibody (ab135865) at 1/500 dilution + HT29 cell lysates at 30 µg

        Predicted band size: 37 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab135865? Please let us know so that we can cite the reference in this datasheet.

      ab135865 has been referenced in 1 publication.

      • Corciulo C  et al. Endogenous adenosine maintains cartilage homeostasis and exogenous adenosine inhibits osteoarthritis progression. Nat Commun 8:15019 (2017). Mouse, Rat . PubMed: 28492224

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Adenosine A2b Receptor/ADORA2B antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (pancreas)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9.0
      Permeabilization
      Yes - Wash Buffer from Dako
      Specification
      pancreas
      Fixative
      Paraformaldehyde
      Read More

      Mr. Rudolf Jung

      Verified customer

      Submitted Sep 06 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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