Adenosine Assay Kit (Fluorometric) (ab211094)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 15 min
- Sample type: Plasma, Urine
Overview
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Product name
Adenosine Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Urine, Plasma -
Assay type
Quantitative -
Assay time
0h 15m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Adenosine Assay Kit (Fluorometric) (ab211094) provides a convenient method to detect adenosine in plasma and urine. In this assay, adenosine is measured using adenosine deaminase followed by a multi-step enzymatic approach resulting in the generation of an intermediate that reacts with the adenosine probe, leading to the formation of a fluorescent product. The fluorescent product can be detected at Ex/Em = 535/587 nm, and its intensity is proportional to the amount of adenosine in the sample.
The detection range of this product is 2-80 pmol of adenosine in plasma or urine.
Adenosine assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 15 min
- analyze with fluorescence microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K327 Adenosine Assay Kit (Fluorometric). K327-100 is the same size as the 100 test size of ab211094.
Adenosine, a purine nucleoside, is present throughout the body. It plays an important role in energy transfer via the formation of ATP, ADP and AMP and in signal transduction via the formation of cAMP. Adenosine mediates its effects directly via adenosine receptors A1, A2A, A2B and A3. It regulates myocardial oxygen consumption and coronary blood flow, exerts anti-inflammatory effects throughout the body and also regulates the Renin-Angiotensin system. It also plays a role in tissue damage and repair, and cell death. Plasma adenosine levels are increased in patients with ischemic and non-ischemic heart failure.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer X 1 x 25ml Converter Enzyme IX 1 vial Adenosine Deaminase I 1 vial Adenosine Developer 1 vial Adenosine Standard 1 x 100µl OxiRed Probe 1 x 200µl Xanthine Enzyme Mix 1 vial
Images
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab211094 has been referenced in 19 publications.
- Saxena V et al. Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses. Cell Rep 39:110727 (2022). PubMed: 35443187
- Wang X et al. Decreased CD73+ Double-Negative T Cells and Elevated Level of Soluble CD73 Correlated With and Predicted Poor Immune Reconstitution in HIV-Infected Patients After Antiretroviral Therapy. Front Immunol 13:869286 (2022). PubMed: 35444646
- Yoshida R et al. MET-Induced CD73 Restrains STING-Mediated Immunogenicity of EGFR-Mutant Lung Cancer. Cancer Res 82:4079-4092 (2022). PubMed: 36066413
- Ehlers L et al. Surface AMP deaminase 2 as a novel regulator modifying extracellular adenine nucleotide metabolism. FASEB J 35:e21684 (2021). PubMed: 34159634
- Medina CB et al. Pannexin 1 channels facilitate communication between T cells to restrict the severity of airway inflammation. Immunity 54:1715-1727.e7 (2021). PubMed: 34283971