Overview

  • Product name

    Adenosine Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Urine, Plasma
  • Assay type

    Quantitative
  • Assay time

    0h 15m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Adenosine Assay Kit (Fluorometric) (ab211094) provides a convenient method to detect adenosine in plasma and urine. In this assay, adenosine is measured using adenosine deaminase followed by a multi-step enzymatic approach resulting in the generation of an intermediate that reacts with the adenosine probe, leading to the formation of a fluorescent product. The fluorescent product can be detected at Ex/Em = 535/587 nm, and its intensity is proportional to the amount of adenosine in the sample.


    The detection range of this product is 2-80 pmol of adenosine in plasma or urine.


    Adenosine assay protocol summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 15 min
    - analyze with fluorescence microplate reader

  • Notes

    Adenosine, a purine nucleoside, is present throughout the body. It plays an important role in energy transfer via the formation of ATP, ADP and AMP and in signal transduction via the formation of cAMP. Adenosine mediates its effects directly via adenosine receptors A1, A2A, A2B and A3. It regulates myocardial oxygen consumption and coronary blood flow, exerts anti-inflammatory effects throughout the body and also regulates the Renin-Angiotensin system. It also plays a role in tissue damage and repair, and cell death. Plasma adenosine levels are increased in patients with ischemic and non-ischemic heart failure.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Adenosine Assay Buffer 1 x 25ml
    Adenosine Convertor (10 U) 1 vial
    Adenosine Detector (10 U) 1 vial
    Adenosine Developer (1 U) 1 vial
    Adenosine Probe (in DMSO) 1 x 200µl
    Adenosine Standard (10 mM) 1 x 100µl
    Urine Clarifier (1 U) 1 vial

Images

  • Typical Adenosine standard calibration curve.

  • Measurement of Adenosine in pooled human plasma (20 µL) and human urine (4 µL of pretreated urine, 2X diluted during the pre-treatment method).

  • Adenosine amount in human plasma and human urine, calculated from data shown in previous figure.

Protocols

References

ab211094 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Assay of cell culture media

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I used this kit to measure release of adenosine from osteoclasts in culture, derived from the PBMC of 2 separate donors. A 1 in 100 dilution of the culture medium gave an adenosine concentration within the range of the kit for both my control and drug-treated cells.

AbTRIAL reference: 180925-001744A

Abcam user community

Verified customer

Submitted Oct 05 2018

Adenosine kit calibrations

Good Average 3/5 (Ease of Use)
Abreviews
I performed calibrations and experiments with cell medium. The calibration took some time and required the use of the entire kit. After I got past that stage, things got much easier.

Dr. Mor Pavlovsky

Verified customer

Submitted Jun 04 2018

Adenosine kit calibrations

Good Excellent 5/5 (Ease of Use)
Abreviews
I performed calibrations and one experiment with mouse brain samples. The brains homogenates were in the buffer that the kit supplies, and centrifuged for 15 min in 4 degrees in 12,700 RPM followed by manufacturer's instructions.


Mrs. Lital Sahar

Verified customer

Submitted Feb 06 2018

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