Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture media, Cell Lysate, Purified protein, Tissue Lysate
- Sensitivity: 10 µU
Product nameAdenosine Deaminase (ADA) Activity Assay Kit (Fluorometric)
See all ADA kits
Sample typeCell Lysate, Cell culture media, Purified protein, Tissue Lysate
Assay typeEnzyme activity
Range10 µU - 100 µU
Species reactivityReacts with: Mammals, Other species
Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (ab204695) is an assay where inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product that can be easily quantified at Ex/Em = 535/587 nm. The kit measures total activity of Adenosine Deaminase with limit of quantification of 10 μU recombinant Adenosine Deaminase. The range of the assay is from 10-100 μU.
Adenosine Deaminase (ADA, EC 220.127.116.11) is an enzyme that catalyses the conversion of adenosine and 2’-deoxyadenosine to inosine and 2’-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and has been found associated with DPP4/CD26 in the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation.
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Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests ADA Assay Buffer (10x) 1 x 25ml ADA Convertor 1 vial ADA Developer 1 vial ADA Positive Control 1 vial ADA Probe 1 x 200µl ADA Substrate 1 x 500µl Inosine Standard (10 mM) Yellow 1 x 100µl
FunctionCatalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
Tissue specificityFound in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
Involvement in diseaseDefects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.
Sequence similaritiesBelongs to the adenosine and AMP deaminases family.
Cellular localizationCell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
- Information by UniProt
ab204695 has been referenced in 2 publications.
- Sánchez-Melgar A et al. Resveratrol Modulates and Reverses the Age-Related Effect on Adenosine-Mediated Signalling in SAMP8 Mice. Mol Neurobiol 56:2881-2895 (2019). PubMed: 30069829
- Netsch P et al. Human mesenchymal stromal cells inhibit platelet activation and aggregation involving CD73-converted adenosine. Stem Cell Res Ther 9:184 (2018). PubMed: 29973267