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Signal Transduction Second Messenger Nucleotide Messenger cAMP
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RecombinantRabMAb

Recombinant Anti-Adenosine phosphate antibody [EPR8471] (ab140604)

This fast track antibody is not yet fully characterized. It is subject to these terms and conditions.
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ELISA - Anti-Adenosine phosphate antibody [EPR8471] (ab140604)
  • Anti-Adenosine phosphate antibody [EPR8471] (ab140604)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8471] to Adenosine phosphate
  • Suitable for: ELISA
  • Reacts with: Species independent

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Adenosine phosphate antibody [EPR8471]
    See all Adenosine phosphate primary antibodies
  • Description

    Rabbit monoclonal [EPR8471] to Adenosine phosphate

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species

    Rabbit
  • Specificity

    ab140604 recognizes cAMP, AMP, ADP, and ATP only. It has less cross-reactivity with cGMP, GMP, GDP,GTP, and Adenosine.
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Adenosine phosphate conjugated to keyhole limpet haemocyanin.

  • Positive control

    • cAMP, AMP, ADP, and ATP
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8471
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP

Associated products

  • Alternative Versions

    • Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (ab228606)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
ELISA
Use a concentration of 0.1 µg/ml.
Notes
ELISA
Use a concentration of 0.1 µg/ml.

Target

  • Relevance

    Cyclic adenosine-3’, 5’-monophosphate (cAMP) is the second messenger in the intracellular signal cascade, which is synthesis from adenosine triphosphate (ATP) by adenylyl cyclase. In the brain, cAMP signaling is involved in a multitude of mechanisms in neurons, astrocytes, oligodendrocytes and microglia. Examples are signal transduction in synapses, communication between neurons and glia cells or inflammatory processes. Alternations of cAMP affect normal brain functions; hence making cAMP a potential biomarkers for Parkinson’s diseases, amyotrophic lateral sclerosis and Creutzfeldt-Jakob disease
  • Alternative names

    • adenosine diphosphate antibody
    • adenosine monophosphate antibody
    • adenosine triphosphate antibody
    • ADP antibody
    • AMP antibody
    • ATP antibody
    • cAMP antibody
    • Cyclic adenosine-3’, 5’-monophosphate antibody
    see all

Images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • ELISA - Anti-Adenosine phosphate antibody [EPR8471] (ab140604)
    ELISA - Anti-Adenosine phosphate antibody [EPR8471] (ab140604)
    50 µl of 0.1ug/ml of cAMP-SPDP-BSA was coated into 96-wells. After blocking with 1% BSA, 25 µl of 5 mg/ml of cAMP, 5’-AMP, 5’-AMP-Na2, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, or H2O and 25 µl of ab140604 at 0.5 ug/ml were added to 96-wells and incubated for 30 min on a shaker. After washing, HRP conjugated goat anti-rabbit IgG Fc specific antibody was added and incubated. After washing, TMB substrate was used to develop color.
  • Anti-Adenosine phosphate antibody [EPR8471] (ab140604)
    Anti-Adenosine phosphate antibody [EPR8471] (ab140604)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab140604? Please let us know so that we can cite the reference in this datasheet.

    ab140604 has not yet been referenced specifically in any publications.

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