Recombinant Anti-Adenosine phosphate antibody [EPR8471] (ab140604)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Adenosine phosphate antibody [EPR8471]
See all Adenosine phosphate primary antibodies -
Description
Rabbit monoclonal [EPR8471] to Adenosine phosphateThis product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Specificity
ab140604 recognizes cAMP, AMP, ADP, and ATP only. It has less cross-reactivity with cGMP, GMP, GDP,GTP, and Adenosine. -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Adenosine phosphate conjugated to keyhole limpet haemocyanin.
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Positive control
- cAMP, AMP, ADP, and ATP
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8471 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use a concentration of 0.1 µg/ml. |
Target
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Relevance
Cyclic adenosine-3’, 5’-monophosphate (cAMP) is the second messenger in the intracellular signal cascade, which is synthesis from adenosine triphosphate (ATP) by adenylyl cyclase. In the brain, cAMP signaling is involved in a multitude of mechanisms in neurons, astrocytes, oligodendrocytes and microglia. Examples are signal transduction in synapses, communication between neurons and glia cells or inflammatory processes. Alternations of cAMP affect normal brain functions; hence making cAMP a potential biomarkers for Parkinson’s diseases, amyotrophic lateral sclerosis and Creutzfeldt-Jakob disease -
Alternative names
- adenosine diphosphate antibody
- adenosine monophosphate antibody
- adenosine triphosphate antibody
see all
Images
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![ELISA - Anti-Adenosine phosphate antibody [EPR8471] (ab140604)](https://www.abcam.com/ps/products/140/ab140604/Images/Adenosine-phosphate-Primary-antibodies-ab140604-1.jpg)
ELISA - Anti-Adenosine phosphate antibody [EPR8471] (ab140604)50 µl of 0.1ug/ml of cAMP-SPDP-BSA was coated into 96-wells. After blocking with 1% BSA, 25 µl of 5 mg/ml of cAMP, 5’-AMP, 5’-AMP-Na2, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, or H2O and 25 µl of ab140604 at 0.5 ug/ml were added to 96-wells and incubated for 30 min on a shaker. After washing, HRP conjugated goat anti-rabbit IgG Fc specific antibody was added and incubated. After washing, TMB substrate was used to develop color.
Datasheets and documents
References
ab140604 has not yet been referenced specifically in any publications.
