Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8471] to Adenosine phosphate - BSA and Azide free
- Suitable for: ELISA
- Reacts with: Species independent
Product nameAnti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free
See all Adenosine phosphate primary antibodies
DescriptionRabbit monoclonal [EPR8471] to Adenosine phosphate - BSA and Azide free
This antibody recognizes cAMP, AMP, ADP, and ATP only. It has less cross-reactivity with cGMP, GMP, GDP,GTP, and Adenosine.
Tested applicationsSuitable for: ELISAmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Adenosine phosphate conjugated to keyhole limpet haemocyanin. KLH conjugated cAMP.
- cAMP, AMP, ADP, and ATP
Ab228606 is the carrier-free version of ab140604. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab228606 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab228606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
RelevanceCyclic adenosine-3’, 5’-monophosphate (cAMP) is the second messenger in the intracellular signal cascade, which is synthesis from adenosine triphosphate (ATP) by adenylyl cyclase. In the brain, cAMP signaling is involved in a multitude of mechanisms in neurons, astrocytes, oligodendrocytes and microglia. Examples are signal transduction in synapses, communication between neurons and glia cells or inflammatory processes. Alternations of cAMP affect normal brain functions; hence making cAMP a potential biomarkers for Parkinson’s diseases, amyotrophic lateral sclerosis and Creutzfeldt-Jakob disease
- adenosine diphosphate antibody
- adenosine monophosphate antibody
- adenosine triphosphate antibody
This data was developed using ab140604, the same antibody clone in a different buffer formulation.
50 µl of 0.1µg/ml of cAMP-SPDP-BSA was coated into 96-wells. After blocking with 1% BSA, 25 µl of 5 mg/ml of cAMP, 5�-AMP, 5�-AMP-Na2, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, or H2O and 25 µl of ab140604 at 0.5 µg/ml were added to 96-wells and incubated for 30 min on a shaker. After washing, HRP conjugated goat anti-rabbit IgG Fc specific antibody was added and incubated. After washing, TMB substrate was used to develop color.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228606 has not yet been referenced specifically in any publications.