Recombinant Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (ab228606)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8471] to Adenosine phosphate - BSA and Azide free
- Suitable for: ELISA
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free
See all Adenosine phosphate primary antibodies -
Description
Rabbit monoclonal [EPR8471] to Adenosine phosphate - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody recognizes cAMP, AMP, ADP, and ATP only. It has less cross-reactivity with cGMP, GMP, GDP,GTP, and Adenosine.
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Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Adenosine phosphate conjugated to keyhole limpet haemocyanin. KLH conjugated cAMP.
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Positive control
- cAMP, AMP, ADP, and ATP
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General notes
ab228606 is the carrier-free version of ab140604.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8471 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Cyclic adenosine-3’, 5’-monophosphate (cAMP) is the second messenger in the intracellular signal cascade, which is synthesis from adenosine triphosphate (ATP) by adenylyl cyclase. In the brain, cAMP signaling is involved in a multitude of mechanisms in neurons, astrocytes, oligodendrocytes and microglia. Examples are signal transduction in synapses, communication between neurons and glia cells or inflammatory processes. Alternations of cAMP affect normal brain functions; hence making cAMP a potential biomarkers for Parkinson’s diseases, amyotrophic lateral sclerosis and Creutzfeldt-Jakob disease -
Alternative names
- adenosine diphosphate antibody
- adenosine monophosphate antibody
- adenosine triphosphate antibody
see all
Images
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This data was developed using ab140604, the same antibody clone in a different buffer formulation.50 µl of 0.1µg/ml of cAMP-SPDP-BSA was coated into 96-wells. After blocking with 1% BSA, 25 µl of 5 mg/ml of cAMP, 5'-AMP, 5'-AMP-Na2, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, or H2O and 25 µl of ab140604 at 0.5 µg/ml were added to 96-wells and incubated for 30 min on a shaker. After washing, HRP conjugated goat anti-rabbit IgG Fc specific antibody was added and incubated. After washing, TMB substrate was used to develop color.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab228606 has not yet been referenced specifically in any publications.