Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)


  • Product name
    Anti-Adenosine Receptor A2a antibody [7F6-G5-A2]
    See all Adenosine Receptor A2a primary antibodies
  • Description
    Mouse monoclonal [7F6-G5-A2] to Adenosine Receptor A2a
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Hamster, Dog, Non human primates
  • Immunogen

    Full length protein corresponding to Human Adenosine Receptor A2a.

  • Epitope
    Recognises amino acids 213-220 (SQPLPGER) within the third intracellular loop.
  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH In western blot, this antibody gave a positive result in SHSY-5Y, SK-N-SH, PC12 and human platelet whole cell lysates.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab79714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).

We recommend using 1% BSA as a blocking agent for western blot.

ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Domain
    The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.
  • Post-translational
    Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A2AAR antibody
    • A2aR antibody
    • AA2AR_HUMAN antibody
    • ADENO antibody
    • Adenosine A2 receptor antibody
    • Adenosine A2a receptor antibody
    • Adenosine receptor A2a antibody
    • Adenosine receptor subtype A2a antibody
    • ADORA 2 antibody
    • ADORA2 antibody
    • ADORA2A antibody
    • hA2aR antibody
    • RDC 8 antibody
    • RDC8 antibody
    see all


  • All lanes : Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714) at 5 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SK-N-SH (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : Platelet (Human) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa

    Exposure time: 2 minutes

    We recommend using 1% BSA as a blocking agent for western blot. Milk is not a suitable blocking agent for this product.

  • ICC/IF image of ab79714 stained SKNSH cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79714 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.


This product has been referenced in:
  • Sakano D  et al. Dopamine D2 Receptor-Mediated Regulation of Pancreatic ß Cell Mass. Stem Cell Reports 7:95-109 (2016). Mouse . Read more (PubMed: 27373926) »
  • Niesman IR  et al. Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice. J Neuroinflammation 11:39 (2014). WB ; Mouse . Read more (PubMed: 24593993) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8)
Human Tissue lysate - whole (Heart)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 10 2013

Thank you for that reply.

Kate is currently away from the office but I will try to help as best I can with your enquiry. I have checked the two secondary antibodies intended to be used and they seem like they should perform fine with the prima...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments.

Before proce...

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Thank you for your message and for kindly confirming these details.

I look forward to hearing from you with the results from the samples lysed in RIPA. Please do not hesitate to let me know how you get on. If this still does not work with RI...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your reply.

I can confirm that I send you the quote for ab79714 in a separate email.

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Immunocytochemistry/ Immunofluorescence
Human Cell (HepG2)
Yes - PBS containing 0.2% Triton X100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Mar 18 2011


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