Overview

  • Product name

    Anti-Adenosine Receptor A2a antibody [7F6-G5-A2]
    See all Adenosine Receptor A2a primary antibodies
  • Description

    Mouse monoclonal [7F6-G5-A2] to Adenosine Receptor A2a
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Hamster, Dog, Non human primates
  • Immunogen

    Full length protein corresponding to Human Adenosine Receptor A2a.
    Database link: P29274

  • Epitope

    Recognises amino acids 213-220 (SQPLPGER) within the third intracellular loop.
  • Positive control

    • This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH In western blot, this antibody gave a positive result in SHSY-5Y, SK-N-SH, PC12 and human platelet whole cell lysates.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab79714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).

We recommend using 1% BSA as a blocking agent for western blot.

ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Domain

    The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.
  • Post-translational
    modifications

    Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A2AAR antibody
    • A2aR antibody
    • AA2AR_HUMAN antibody
    • ADENO antibody
    • Adenosine A2 receptor antibody
    • Adenosine A2a receptor antibody
    • Adenosine receptor A2a antibody
    • Adenosine receptor subtype A2a antibody
    • ADORA 2 antibody
    • ADORA2 antibody
    • ADORA2A antibody
    • hA2aR antibody
    • RDC 8 antibody
    • RDC8 antibody
    see all

Images

  • ICC/IF image of ab79714 stained SKNSH cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79714 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714) at 5 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SK-N-SH (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : Platelet (Human) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa


    Exposure time: 2 minutes


    We recommend using 1% BSA as a blocking agent for western blot. Milk is not a suitable blocking agent for this product.

References

This product has been referenced in:

  • Sakano D  et al. Dopamine D2 Receptor-Mediated Regulation of Pancreatic ß Cell Mass. Stem Cell Reports 7:95-109 (2016). Mouse . Read more (PubMed: 27373926) »
  • Niesman IR  et al. Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice. J Neuroinflammation 11:39 (2014). WB ; Mouse . Read more (PubMed: 24593993) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Human Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 10 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2)
Specification
HepG2
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS containing 0.2% Triton X100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Mar 18 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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