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Lot number: GR80530-1
Inquiry: Hi Technical, We have a customer who has experiencing problems with this antibody. See attached a questionnaire. Kind regards
Asked on Sep 04 2012
Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.
I would like to reassure you that ab79714 is tested and covered by our 6 month guarantee for use in WB and human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.
Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:
1. I notice the primary antbody ab79714 is a mouse monoclonal IgG2a antibody. The secondary antibody mentioned on the form is goat anti rabbit, which will not detect the mouse primary. I can suggest to use a secondary antibody which is anti mouse IgG, or anti mouse IgG2a.
2. I can recommend to try RIPA lysis buffer to prepare the samples. This may provide amore suitable protein preparation.
3. What were the results from the loading control (GAPDH and b actin)?
I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.
Answered on Sep 04 2012