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Thank you for your patience.
Our customer has responded with the following;
In regard to your questions:
1. My mistake I actually meant we used an anti-mouse secondary not anti-rabbit.
2. I will run our next lot of samples using both our standard buffer and the RIPA buffer and will email you the results once we try them with the new antibodies.
3. We can routinely detect ng quantities of both GAPDH and b-actin as our controls - so we did not think it was anything to do with sample preparation.
Looking forward to your response.
Asked on Sep 24 2012
Thank you for your message and for kindly confirming these details.
I look forward to hearing from you with the results from the samples lysed in RIPA. Please do not hesitate to let me know how you get on. If this still does not work with RIPA, I'll be pleased to arrange a free of charge replacement or credit note.
I hope this will be helpful.
Answered on Sep 24 2012