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Our customer has responded with the following;
In regards to the query from Abcam the secondary antibody that we have been using to detect A2A using ab79714 is an Alexa Fluor 700 Goat-anti-mouse IgG2 (H+L) from xxxxx
If we are able to get a replacement for this using the alternative ab101678 we will be using Alexa Fluor 700 Goat-anti-rabbit IgG2 (H+L) from xxxxx
The good news is the replacement antibody for the mGluR5 is working perfectly on human brn extracts and we are detecting a single band at 140 kDa.
Please let me know if you have any further questions.
Looking forward to your response.
Asked on Oct 23 2012
Thank you for that reply.
Kate is currently away from the office but I will try to help as best I can with your enquiry. I have checked the two secondary antibodies intended to be used and they seem like they should perform fine with the primary antibodies. As Kate has indicated, I would be happy to provide the alternative antibody ab101678 if your customer would like.
Could you please just confirm I have the correct order number:
Then I will arrange for the replacement to be sent to you as soon as possible.
I look forward to receiving your reply.
Answered on Oct 23 2012