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Anti-Adenosine Receptor A2a antibody (ab3461)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (27)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)

Key features and details

  • Rabbit polyclonal to Adenosine Receptor A2a
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Adenosine Receptor A2a antibody
    See all Adenosine Receptor A2a primary antibodies
  • Description

    Rabbit polyclonal to Adenosine Receptor A2a
  • Host species

    Rabbit
  • Specificity

    Detects adenosine receptor A2a. This antibody does not detect other AR subtypes.
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse
  • Immunogen

    Synthetic peptide corresponding to Dog Adenosine Receptor A2a aa 373-391.
    Sequence:

    ESHGDMGLPDVELLSHELK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human testis and placenta tissues. ICC: U251 and SKNSH cells. WB: HepG2 and HeLa cell lysates; Human placenta tissue lysate; Mouse liver tissue lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Cell Biology
    • Apoptosis
    • Phagocytosis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Phagocytosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Adenosine Receptor A2a protein (ab126024)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB (1)
1/1000.
IHC-P
1/20 - 1/200.
Notes
ICC
Use at an assay dependent concentration.
WB
1/1000.
IHC-P
1/20 - 1/200.

Target

  • Function

    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Domain

    The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.
  • Post-translational
    modifications

    Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P29274 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 135 Human
    • Entrez Gene: 11540 Mouse
    • Omim: 102776 Human
    • SwissProt: Q6TLI7 Horse
    • SwissProt: P29274 Human
    • SwissProt: Q60613 Mouse
    • Unigene: 197029 Human
    • Unigene: 333734 Mouse
    • Alternative names

      • A2AAR antibody
      • A2aR antibody
      • AA2AR_HUMAN antibody
      • ADENO antibody
      • Adenosine A2 receptor antibody
      • Adenosine A2a receptor antibody
      • Adenosine receptor A2a antibody
      • Adenosine receptor subtype A2a antibody
      • ADORA 2 antibody
      • ADORA2 antibody
      • ADORA2A antibody
      • hA2aR antibody
      • RDC 8 antibody
      • RDC8 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)

      ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
      Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
      All lanes : Anti-Adenosine Receptor A2a antibody (ab3461) at 1/500 dilution

      Lane 1 : Human placenta cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Mouse liver cell lysate

      Lysates/proteins at 25 µg per lane.
    • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)

      Immunocytochemistry/Immunofluorescence analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3461 in 3% BSA-PBS at a dilution of 1:20 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)

      ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)

      ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
      ICC/IF image of ab3461 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3461, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (27)

    Publishing research using ab3461? Please let us know so that we can cite the reference in this datasheet.

    ab3461 has been referenced in 27 publications.

    • Li D  et al. Gut microbiota-derived inosine from dietary barley leaf supplementation attenuates colitis through PPAR? signaling activation. Microbiome 9:83 (2021). PubMed: 33820558
    • Yang D  et al. Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathway. Elife 10:N/A (2021). PubMed: 33416495
    • Jin H  et al. Increased Extracellular Adenosine in Radiotherapy-Resistant Breast Cancer Cells Enhances Tumor Progression through A2AR-Akt-ß-Catenin Signaling. Cancers (Basel) 13:N/A (2021). PubMed: 33925516
    • Bhalla M  et al. A1 adenosine receptor signaling reduces Streptococcus pneumoniae adherence to pulmonary epithelial cells by targeting expression of platelet-activating factor receptor. Cell Microbiol 22:e13141 (2020). PubMed: 31709673
    • Lisztes E  et al. Adenosine Promotes Human Hair Growth and Inhibits Catagen Transition In Vitro: Role of the Outer Root Sheath Keratinocytes. J Invest Dermatol 140:1085-1088.e6 (2020). PubMed: 31730764
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    ELISA abreview for Anti-Adenosine Receptor A2a antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Cell (Cell Membrane)
    Specification
    Cell Membrane
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Sandwich (Detection)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Tammy Zhai

    Verified customer

    Submitted Apr 22 2015

    Western blot

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Human Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 10 2013

    Question

      A researcher from Mexico needs to use a Anti-Adenosine Receptor A2a antibody in rat and CoIP. I found ab3461 but it has not been tested for rat. Could you check the homology to see if you would recommend trying this antibody and if the 100% testing discount would apply. He also needs Anti-Dopamine D2 Receptor antibody to use in rat and CoIP. I found ab21218 that has been tested in rat and IP, but he wants to know if it will work in CoIP as well.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2011

    Answer

    Hi Daniela, I did an alignment between the rat sequence and the immnunogen sequence for ab3461 and I would not recommend using this antibody in rat. The level of similarity between the two is to low. I would however recommend ab101678, it has not been tested in IP, but it will react with rat tissue. The customer could get a testing discount for trying it out in IP. As for ab21218, I think that one should work in CoIP, if it works in IP, it should work for CoIP. Let me know if there is anything else I can help with.  

    Read More

    Abcam Scientific Support

    Answered on Oct 26 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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