Anti-Adenosine Receptor A2a antibody (ab3461)
Key features and details
- Rabbit polyclonal to Adenosine Receptor A2a
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG
Overview
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Product name
Anti-Adenosine Receptor A2a antibody
See all Adenosine Receptor A2a primary antibodies -
Description
Rabbit polyclonal to Adenosine Receptor A2a -
Host species
Rabbit -
Specificity
Detects adenosine receptor A2a. This antibody does not detect other AR subtypes. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human
Predicted to work with: Horse
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Immunogen
Synthetic peptide corresponding to Dog Adenosine Receptor A2a aa 373-391.
Sequence:ESHGDMGLPDVELLSHELK
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab3461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. | |
| IHC-P | 1/20 - 1/200. |
Target
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Function
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Domain
The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface. -
Post-translational
modificationsUbiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 135 Human
- Entrez Gene: 11540 Mouse
- Entrez Gene: 25369 Rat
- Omim: 102776 Human
- SwissProt: P11617 Dog
- SwissProt: Q6TLI7 Horse
- SwissProt: P29274 Human
- SwissProt: Q60613 Mouse
see all -
Alternative names
- A2AAR antibody
- A2aR antibody
- AA2AR_HUMAN antibody
see all
Images
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Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)All lanes : Anti-Adenosine Receptor A2a antibody (ab3461) at 1/500 dilution
Lane 1 : Human placenta cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Mouse liver cell lysate
Lysates/proteins at 25 µg per lane. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)This image is courtesy of an anonymous AbreviewAnti-Adenosine Receptor A2a antibody (ab3461) at 1/1000 dilution + Human heart tissue lysate at 20 µg
Secondary
Donkey HRP-conjugate anti-rabbit at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutes
Datasheets and documents
References
ab3461 has been referenced in 17 publications.
- Gordian E et al. Transforming growth factor ß-induced epithelial-to-mesenchymal signature predicts metastasis-free survival in non-small cell lung cancer. Oncotarget 10:810-824 (2019). PubMed: 30783512
- Wiltberger G et al. Protective effects of coffee consumption following liver transplantation for hepatocellular carcinoma in cirrhosis. Aliment Pharmacol Ther 49:779-788 (2019). PubMed: 30811647
- Han YY et al. Cordycepin improves behavioral-LTP and dendritic structure in hippocampal CA1 area of rats. J Neurochem 151:79-90 (2019). PubMed: 31314908
- Xu K et al. Adenosine from a biologic source regulates neutrophil extracellular traps (NETs). J Leukoc Biol 105:1225-1234 (2019). PubMed: 30907983
- Márquez-Gómez R et al. Functional histamine H3 and adenosine A2A receptor heteromers in recombinant cells and rat striatum. Pharmacol Res 129:515-525 (2018). PubMed: 29217157
