Recombinant Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Product name

Anti-Adenosine Receptor A2a antibody [EPR23731-50]
See all Adenosine Receptor A2a primary antibodies
Description

Rabbit monoclonal [EPR23731-50] to Adenosine Receptor A2a
Host species

Rabbit
Specificity

Expression levels of the target protein vary with sample type and optimisation may be required. In our hands we are only able to detect ADORA2A in relevant neurological samples and do not recommend this product for use in other samples.
Tested applications

Suitable for: IHC-Pmore details
Unsuitable for: IP or WB
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human cerebrum tissue; Mouse cerebrum tissue; Rat cerebrum tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23731-50
Isotype

IgG
Research areas
- Neuroscience
- Processes

Alternative Versions
- Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Related Products
- Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab260032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application notes

Is unsuitable for IP or WB.

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Sequence similarities

Belongs to the G-protein coupled receptor 1 family.
Domain

The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.
Post-translational
modifications

Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P29274 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 135 Human
- Entrez Gene: 11540 Mouse
- Entrez Gene: 25369 Rat
- Omim: 102776 Human
- SwissProt: P29274 Human
- SwissProt: Q60613 Mouse
- SwissProt: P30543 Rat
- Unigene: 197029 Human
- Unigene: 333734 Mouse
- Unigene: 11180 Rat
see all
Alternative names
- A2AAR antibody
- A2aR antibody
- AA2AR_HUMAN antibody
- ADENO antibody
- Adenosine A2 receptor antibody
- Adenosine A2a receptor antibody
- Adenosine receptor A2a antibody
- Adenosine receptor subtype A2a antibody
- ADORA 2 antibody
- ADORA2 antibody
- ADORA2A antibody
- hA2aR antibody
- RDC 8 antibody
- RDC8 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Immunohistochemical analysis of paraffin-embedded human cerebrum (striatum) tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum is observed. The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilutionfollowed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the striatum of the mouse cerebrum (PMID: 21858817). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Negative control: No staining observed on human diffuse large B-cell lymphoma tissue .

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Negative control: No staining observed on human hepatocellular carcinoma tissue.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Negative control: No staining observed on human spleen tissue.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Immunohistochemical analysis of paraffin-embedded human thymoma tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Negative control: No staining observed on human thymoma tissue.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Tissue Microarrays stained for "Anti-Adenosine Receptor A2a antibody [EPR23731-50]” using "ab260032" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab260032 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

Tissue Microarrays stained for "Anti-Adenosine Receptor A2a antibody [EPR23731-50]” using "ab260032" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab260032 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab260032? Please let us know so that we can cite the reference in this datasheet.

ab260032 has been referenced in 1 publication.

Sohn R et al. Anti-Inflammatory Effects of Endogenously Released Adenosine in Synovial Cells of Osteoarthritis and Rheumatoid Arthritis Patients. Int J Mol Sci 22:N/A (2021). PubMed: 34445661

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (1)

Customer reviews and Q&As

Post-translational
modifications