Recombinant
RabMAb

Anti-Adenosyl Homocysteine antibody [EPR4499] (ab111903)

Submit an Abreview Q&A (10)Specific References (1)

Overview

  • Product name
    Anti-Adenosyl Homocysteine antibody [EPR4499]
    See all Adenosyl Homocysteine primary antibodies
  • Description
    Rabbit monoclonal [EPR4499] to Adenosyl Homocysteine
  • Host species
    Rabbit
  • Specificity
    ab111903 shows the following reactivities with related compounds: S-(5'-Adenosyl)-L-homocysteine: 100% S-(5'-Adenosyl)-L-methionine: 0% Adenosine: 4.4% Homocysteine: 4.4% Cystathionine: 0% L-Cysteine: 1.5% Glutathione: 1.8%
  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Immunogen

    Adenosyl Homocysteine conjugated to KLH.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab111903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA 1/5000.

Target

  • Relevance
    Adenosyl Homocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependant methyl transferase reactions and therefore may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • S (5' Adenosyl) L homocysteine antibody
    • S Adenosyl L homocysteine antibody
    • S adenosylhomocysteine antibody
    • SAH antibody

Images

  • Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] (ab111903)
    Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] (ab111903)
    Competitive ELISA: 0.1 µg/ml of BSA-S-adenosylhomocysteine was coated into 96 wells. Serial dilution of S-adenosylhomocysteine (SAH), SAMe, et al. and 0.05 µg/ml of ab111903 were added. HRP conjugated Goat anti-Rabbit IgG antibody was used to develop the color.

References

This product has been referenced in:
  • Wu C & Tzertzinis G Selection of a mimotope peptide of S-adenosyl-L-homocysteine and its application in immunoassays. Molecules 18:13020-6 (2013). ELISA . Read more (PubMed: 24145794) »

See 1 Publication for this product

Publishing research using ab111903? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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What is the competitive ELISA protocol used with ab111903?

The competitive ELISA protocol is as follow:

1. 0.1 ug/ml of BSA-S-adenosylhomocysteine was coated into 96 wells.50ul/well,4 ℃，overnight.

2. Add 120µl/well of 1% BSA-TBS and incubate for 1 hours at RT.

3.Wash the plate...

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To Whom It May Concern,
I ordered this antibody for a fluorescent polarization assay without realizing the concentration of the Ab is undetermined. Is there an estimated concentration value available? Even a rough range would help me to getting a ...

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Thank you for your enquiry.

I can confirm that ab111903 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated ...

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Anti-Adenosyl Homocysteine antibody

I meant to add that if you would be able to explain a little more fully what it is you are studying and what you are trying to achieve, I may be able to help you with other ideas of how to achieve it.
I look forward to your reply.

Hi

As well as pursuing the ELISA assay, I'm now looking at alternative methodologies for assaying this molecular and would like to try flow cytometry. So I was wondering what approach Abcam would take to validate and optimise and ant...

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I have had a chat to my colleague who is very experienced with flow cytometry in regards to how to optimise these antibodies for this application.
Unfortunately, neither of these antibodies have been used for this application previously and optimi...

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I have two questions about the Anti-Adenosyl Homocysteine antibody (ab111903). What is the concentration of this antibody?  Can I purify this antibody by protein G affinity column? Thanks.

Thank you for your enquiry.

I can confirm that Anti-Adenosyl Homocysteine antibody (ab111903) is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will n...

Read More

Hi xxxxx,

Apologises for the delay, I was away on Friday and tied up with interviews yesterday. Please find below the answers to the ELISA questionnaire

xxxxx

1) Abcam product code ab 111903

2) Abcam order re...

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Thank you for providing that extra information. It is unfortunate that the ab111903 has not out-performed ab35164 so far.

Do you see any signal at all with ab111903? The only thing I can suggest having looked at your protocol and taking into...

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Hi xxxx

Thank you for the Rabbit anti adenosyl homocysteine antibody(ab111903). I am however experiencing some problems. When using it with my initial secondary antibody or your own Goat anti rabbit HRP antibody I’m not seeing any binding. I...

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Sorry for the delay in getting back to you.


I am very sorry to hear that the ab111903 has not performed as well as ab35164. In order to try and understand what is going on would you please be able to send me some protocol details again....

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Dear xxxx

I am now at the point where I need to think about ordering some more antibody. I am seeing improvements in the system but there is still room for more improvement. I would be interested in trying the alternative antibody that you m...

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I'm glad you have seen improvements in using ab35164. May I ask, what do you think has lead to these improvements? It would be very useful information to know in order to advise customers in the future.


Unfortunately due to the size of ...

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I have a question on the Anti-Adenosyl Homocysteine antibody (ab111903) .

The data sheet describe the antibody does not react with SAMe (see attachment). However the figure in the data sheet shows some cross-reaction between the antibod...

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Thank you for your enquiry.

You are correct that there is moderate cross-reactivity to SAMe. I'd put this in the range of 1-4%. I apologize for the error and will have the datasheet updated.

I hope this is helpful. Please contact me...

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1) Abcam product code ab 35164

2) Abcam order reference number or product batch number:-

Lot number GR28951-2

3) Description of the problem

Lack of sensitivity. Low displacement by antigen within sample or st...

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Thank you for the extra details you provided. In answer to your questions:

1. Could you possibly tell which supplier of SAH you used for standards when testing your antibody, as we have discovered that it may be susceptible to oxidation in bo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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