Anti-Adenosyl Homocysteine antibody [EPR4499] (ab111903)


  • Product name
    Anti-Adenosyl Homocysteine antibody [EPR4499]
    See all Adenosyl Homocysteine primary antibodies
  • Description
    Rabbit monoclonal [EPR4499] to Adenosyl Homocysteine
  • Host species
  • Specificity
    ab111903 shows the following reactivities with related compounds: S-(5'-Adenosyl)-L-homocysteine: 100% S-(5'-Adenosyl)-L-methionine: 0% Adenosine: 4.4% Homocysteine: 4.4% Cystathionine: 0% L-Cysteine: 1.5% Glutathione: 1.8%
  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Immunogen

    Adenosyl Homocysteine conjugated to KLH.

  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab111903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA 1/5000.


  • Relevance
    Adenosyl Homocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependant methyl transferase reactions and therefore may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • Cellular localization
  • Alternative names
    • S (5' Adenosyl) L homocysteine antibody
    • S Adenosyl L homocysteine antibody
    • S adenosylhomocysteine antibody
    • SAH antibody


  • Competitive ELISA: 0.1 µg/ml of BSA-S-adenosylhomocysteine was coated into 96 wells. Serial dilution of S-adenosylhomocysteine (SAH), SAMe, et al. and 0.05 µg/ml of ab111903 were added. HRP conjugated Goat anti-Rabbit IgG antibody was used to develop the color.


This product has been referenced in:
  • Wu C & Tzertzinis G Selection of a mimotope peptide of S-adenosyl-L-homocysteine and its application in immunoassays. Molecules 18:13020-6 (2013). ELISA . Read more (PubMed: 24145794) »

See 1 Publication for this product

Customer reviews and Q&As

The competitive ELISA protocol is as follow:

1. 0.1 ug/ml of BSA-S-adenosylhomocysteine was coated into 96 wells.50ul/well,4 ℃,overnight.

2. Add 120µl/well of 1% BSA-TBS and incubate for 1 hours at RT.

3.Wash the plate...

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Thank you for your enquiry.

I can confirm that ab111903 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated ...

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I meant to add that if you would be able to explain a little more fully what it is you are studying and what you are trying to achieve, I may be able to help you with other ideas of how to achieve it.
I look forward to your reply.

I have had a chat to my colleague who is very experienced with flow cytometry in regards to how to optimise these antibodies for this application.
Unfortunately, neither of these antibodies have been used for this application previously and optimi...

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Thank you for your enquiry.

I can confirm that Anti-Adenosyl Homocysteine antibody (ab111903) is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will n...

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Thank you for providing that extra information. It is unfortunate that the ab111903 has not out-performed ab35164 so far.

Do you see any signal at all with ab111903? The only thing I can suggest having looked at your protocol and taking into...

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Sorry for the delay in getting back to you.

I am very sorry to hear that the ab111903 has not performed as well as ab35164. In order to try and understand what is going on would you please be able to send me some protocol details again....

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I'm glad you have seen improvements in using ab35164. May I ask, what do you think has lead to these improvements? It would be very useful information to know in order to advise customers in the future.

Unfortunately due to the size of ...

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Thank you for your enquiry.

You are correct that there is moderate cross-reactivity to SAMe. I'd put this in the range of 1-4%. I apologize for the error and will have the datasheet updated.

I hope this is helpful. Please contact me...

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Thank you for the extra details you provided. In answer to your questions:

1. Could you possibly tell which supplier of SAH you used for standards when testing your antibody, as we have discovered that it may be susceptible to oxidation in bo...

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