Adenosylhomocysteinase (AHCY) Inhibitor Assay Kit (Fluorometric) (ab204694)

Overview

  • Product name

    Adenosylhomocysteinase (AHCY) Inhibitor Assay Kit (Fluorometric)
    See all Adenosylhomocysteinase kits
  • Detection method

    Fluorescent
  • Sample type

    Inhibitor compounds
  • Assay type

    Enzyme activity
  • Product overview

    Adenosylhomocysteinase (AHCY) Inhibitor Screning Kit (Fluorometric) (ab204694) is a simple assay to study, screen or characterize potential inhibitors of AHCY. In this assay, the homocysteine generated from the breakdown of S-Adenosyl Homocysteine (SAH) is measured using a Thiol Detecting Reagent which results in enhanced fluorescence that can be measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible, and therefore Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.

  • Notes

    Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies).

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    3-Deazaneplanocin A (10 μM) (in DMSO) 1 x 10µl
    Adenosine Deaminase 1 vial
    AHCY Assay Buffer 1 x 25ml
    AHCY Enzyme 1 vial
    AHCY Reconstitution Buffer 1 x 250µl
    AHCY Substrate (in DMSO) 1 x 100µl
    Thiol Detecting Reagent (in DMSO) 1 x 200µl
  • Function

    Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • Pathway

    Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
  • Involvement in disease

    Hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency
  • Sequence similarities

    Belongs to the adenosylhomocysteinase family.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Alternative names

    • Adenosylhomocysteinase
    • AdoHcyase
    • ahcY
    • S-adenosyl-L-homocysteine hydrolase
    • SAHH_HUMAN
    see all

Images

  • Inhibition of Adenosylhomocysteinase (AHCY) Enzyme activity by 3-Deazaneplanocin A. IC50 of 3-Deazaneplanocin A was determined to be 0.137 nM. 

Protocols

References

ab204694 has not yet been referenced specifically in any publications.

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