Overview

  • Product name
    Anti-Adenovirus hexon protein antibody [8C4]
    See all Adenovirus hexon protein primary antibodies
  • Description
    Mouse monoclonal [8C4] to Adenovirus hexon protein
  • Host species
    Mouse
  • Specificity
    Not tested with adenovirus type 5.
  • Tested applications
    Suitable for: Sandwich ELISA, ELISA, Immunodiffusion, Flow Cyt, ICCmore details
  • Immunogen

    Hexon antigen of human adenovirus

  • Positive control
    • adenovirus-infected cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    8C4
  • Isotype
    IgG2a
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [1E11] to Adenovirus hexon protein (ab8251). Can be paired with Adenovirus hexon protein antibody [1E11] (ab8251).
ELISA Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.
Flow Cyt Use 1µg for 105 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC Use at an assay dependent concentration.

Target

  • Relevance
    Hexon protein is a major coat protein of adenoviruses. Adenoviruses capsids have three principal protein components: the hexon, the penton, and the fiber. Hexon consists of three subunits together forming two major components of different morphological symmetry. A triangular top with three towers of density is superimposed on a more bulky pseudo hexagonal base. The symmetry of the top is in accord with the trimeric nature of hexon, but that of the base derives from the molecular function, which is to provide a densely packed impenetrable protective outer layer for the virion.
  • Cellular localization
    Major coat protein of adenoviruses.
  • Alternative names
    • Hexon protein antibody
    • Late protein 2 antibody
    • PII antibody

Images

  • Ab8249 (1/1000) positively staining methanol fixed Hek293 cells infected with Adenovirus serotype 5.

    Secondary: Goat anti mouse HRP (1/1000), incubation was carried out for 1 hr at room temperature.

    Detection system: DAB

    Negative Control: Non transfected Hek293 cells

    This image is courtesy of an Abreview submitted on 25 October 2005. We do not have any further information relating to this image.

    See Abreview

References

This product has been referenced in:
  • Dong W  et al. ORCA-010, a novel potency-enhanced oncolytic adenovirus, exerts strong antitumor activity in preclinical models. Hum Gene Ther 25:897-904 (2014). Read more (PubMed: 25093639) »
  • Hsieh JL  et al. Transthyretin-driven oncolytic adenovirus suppresses tumor growth in orthotopic and ascites models of hepatocellular carcinoma. Cancer Sci 100:537-45 (2009). WB . Read more (PubMed: 19134007) »
See all 3 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Human Cultured Cells (hAdenovirus 48 Infected HEK293s)
Permeabilization
No
Specification
hAdenovirus 48 Infected HEK293s
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Feb 03 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human infected cell lines)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako low pH buffer
Permeabilization
No
Specification
Human infected cell lines
Blocking step
Dako Flex peroxidase block as blocking agent for 5 minute(s) · Concentration: 100%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 24 2015

Answer

Thank you for your inquiry.

Please find in the attachment the CoC as requested for this lot.

I am sorry, I could however not find whether and why this product has been previously recommended to be stored at 4°C.

The customer can continue to store this product at 4°C if he wishes. The expected storage time at 4°C is one year, whereas at -20°C the expected storage time is 5 years. For this reason I would therefore recommend the customer to store some aliquots of the product at -°20C if he does not think to use it all in one year and just to keep the working aliquot at 4°C.

I hope this information is helpful. Please do let me know if you have any questions or concerns in this regards.

Read More

Answer

Thank you for your enquiry. Please find attached the CoCs you requested. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 6 months from the date of purchase when stored correctly. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.

Read More

Answer

Thank you for contacting us with your questions. We currently have lots *** of ab8249 available in our UK stock. While we don't assign specific expiration dates for our products, if stored at 4C as indicated on the datasheet, we would expect ab8249 to be viable for up to one year. Our Abpromise guarantees that all of our products will work as stated on the datasheets for up to 6 months after purchase. I hope this information is useful, but please let me know if you have further questions and I'll be happy to help.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (human Adenovirus serotype 5 in HEK293 cells)
Specification
human Adenovirus serotype 5 in HEK293 cells
Fixative
Methanol
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1%

Abcam user community

Verified customer

Submitted Oct 25 2005

Answer

Thank you for your enquiry. Unfortunately I do not have details of the degree of reactivity of ab8249 and ab8251 against the Adenovirus subclasses. However, I can tell you that ab7428 recognises all Adenovirus serotypes. All antibodies are yet to be tested by neutralisation. All tested applications are specified on Abcam product datasheets. At Abcam we are always interested in determining new applications of our antibodies. Therefore I would like to offer you a discount of 20% off the price of ab8249 in exchange for a review of this antiserum by neutralisation. Please e-mail me if you are interested and I will supply you with a unique discount code that you can enter during the ordering process. I look forward to hearing from you.

Read More

Answer

Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody has been tested for ELISA, Immunodiffusion applications but NOT for immunohistochemical staining. We would suggest performing a preliminary experiment and test different dilutions (1/100-1/2000) and then adjust the dilution according to the results. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

Read More

Answer

Thank you for your enquiry. Ab8249 has not been tested in other applications than ELISA or immunodiffusion I'm afraid. This doesn't mean the antibody doesn't work in WB, but that as we haven't tested this application we cannot garantee it will work. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Read More

Answer

Thank you for your enquiry. The clone 8C4 is adenovirus hexon specific. The antibody is directed against a genus-specific hexon antigen, which is present in all human serotypes. Unfortunately we have not tested recognition of AB5 and AB35.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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