Overview

  • Product name
    Anti-Adenovirus Type 2 antibody
    See all Adenovirus Type 2 primary antibodies
  • Description
    Goat polyclonal to Adenovirus Type 2
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with Adenovirus Type 2. No reactivity with RSV, Influenza A & B or Para 1-3. Not yet tested in other species.
  • Immunogen

    Adenovirus, Type 2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/11 000.
    WB: Use at an assay dependent dilution.

    The Western blot displays a multiple band pattern. This is due to the reactivity not only with the hexon, but also with the other structural proteins associated with the hexon to make up the assembly. Bands at ~28, 29, 32, 35, 45, 50, 63, 65, 74, 82 kD and possibly more could be observed.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      More than 100 serologically distinct types of adenovirus have been identified, including 49 types that infect humans. Several of these cause respiratory and conjunctival diseases. Types 1 and 2 constitute 60 percent of isolates from adenovirus infected children.
    • Alternative names
      • Ad2 antibody
      • Adenovirus 2 antibody

    References

    ab20538 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for you reply.

    Your credit note ID is XXXXX.

    If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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    Answer

    Thank you for your reply.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty kit.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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    Question

    Inquiry: 1. Order details: - Batch number: XXXXX - Po: XXXXXX- Abcam product code: ab20538 - Antibody storage conditions (temperature/reconstitution etc) -20℃ 2. Please describe the problem (high background, wrong band size, more bands, no band etc). More bands 3. On what material are you testing the antibody in WB? - Species: insect cell - What’s cell line or tissue SF9 cell - Cell extract or Nuclear extract: Cell extract - Purified protein or Recombinant protein: Recombinant protein 3. The lysate - How much protein was loaded: 10ul - What lysis buffer was used: sample dye - What protease inhibitors were used: no - What loading buffer was used: - Phosphatase inhibitors - Did you heat the samples: temperature and time: 10 min 4. Electrophoresis/Gel conditions/ Transfer conditions - Reducing or non reducing gel: - Reducing agent: - Gel percentage : - Transfer conditions: (Type of membrane, Protein transfer verified): 350 mA 5. Blocking conditions - Buffer: TBS - Blocking agent: milk, BSA, serum, what percentage: milk - Incubation time:1 hour - Incubation temperature: room temperature 6. Primary Antibody - Species: goat - Reacts against: - At what dilution(s) have you tested this antibody:1:1000 - What dilution buffer was used: 5% milk in TBST - Incubation time: overnight - Incubation temperature: 4℃ - What washing steps were done: 4 time,10min 7. Secondary Antibody - Species: rabbit - Reacts against: goat - At what dilution(s) have you tested this antibody: 1:5000 - Incubation time: 1 hour - Wash steps: 3 time,10min - Fluorochrome or enzyme conjugate: HRP - Do you know whether the problems you are experiencing come from the secondary? 8. Detection method ECl, ECl+, other detection method: ECl 9. Did you apply positive and negative controls along with the samples? Please specify. Negative controls, SF9 cell 10. Optimization attempts - How many times have you tried the Western? 4 time - Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control): no - Do you obtain the same results every time e.g. are background bands always in the same place? yes - What steps have you altered? dilution antibody 1:1000 or 1:2000

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    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    According to our records, the Western blot for this antibody displays a multiple band pattern. This is due to the reactivity not only with the hexon, but also with the other structural proteins associated with the hexon to make up the assembly. Bands at ˜28, 29, 32, 35, 45, 50, 63, 65, 74, 82 kD and possibly more could be observed.

    However, I am happy to give some suggestions that may help optimize the results from this antibody:

    -In order to avoid protein degradation products, and therefore lower MW bands, it is crucial to use phosphatase and protease inhibitors in the sample preparation. I would also suggest using RIPA buffer to lysate the cell preparation.

    -We recommend using reducing conditions (boiling and using a reducing agent such as BME or SDS) unless otherwise stated on the datasheet. This will ensure the protein is in the correct conformation to run at the correct Molecular Weight and for the antibody to run.

    -As stated in the datasheet, the storage instructions indicate to add glycerol to a final volume of 50%.

    -Please make sure the secondary antibody works properly with other primaries, in order to dismiss secondary antibody failure.

    I hope this information is helpful. Please do not hesitate to contact us for further assistance.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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