Overview

  • Product name
    Anti-Adenovirus Type 2 antibody
    See all Adenovirus Type 2 primary antibodies
  • Description
    Goat polyclonal to Adenovirus Type 2
  • Host species
    Goat
  • Specificity
    This antibody does not cross-react with parainfluenza 1-3, Influenza. A and B, or RSV.
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with Adenovirus. Does not cross-react with Parainfluenza 1-3, Influenza A or B, or RSV. Not yet tested in other species.
  • Immunogen

    Hexon from adenovirus, type 2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    More than 100 serologically distinct types of adenovirus have been identified, including 49 types that infect humans. Several of these cause respiratory and conjunctival diseases. Types 1 and 2 constitute 60 percent of isolates from adenovirus infected children.
  • Alternative names
    • Ad2 antibody
    • Adenovirus 2 antibody

References

ab24155 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. To answer your question. Yes, this antibody can be used by ELISA. It has been purified using Protein G and therefore should contain just Goat IgG. I would recommend performing the sandwich ELISA protocol as follows, coating the plate as described. SANDWICH ELISA PROTOCOL Buffers and Reagents: For accurate quantitative results, always compare signal of unknown samples against those of a standard curve. Standards (duplicates or triplicates) and blank must be run with each plate to ensure accuracy. Incubation with Primary and Secondary antibody 1. Add 100 µl of diluted primary antibody to each well (10 µg/mL in PBS). 2. Cover the plate with an adhesive plastic and incubate for 2 h at room temperature. 3. Wash the plate four times with PBS. 3. Add 100µl of appropriately diluted samples to relevant wells. Ensure that appropriately diluted standards are included. Samples or standards should preferably be run in triplicate. Incubate 90 minutes at 37oC. (Dilute samples and standards in PBS). Wash x4 in wash buffer. 4. Add 100µl of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. 5. Cover the plate with an adhesive plastic and incubate for 1-2 h at room temperature. 6. Wash the plate four times with PBS. Detection Although many different types of enzymes have been used for detection, horse radish peroxidase (HRP) and alkaline phosphatase (ALP) are the two widely used enzymes employed in ELISA assay. It is important to consider the fact that some biological materials have high levels of endogenous enzyme activity (such as high ALP in alveolar cells, high peroxidase in red blood cells) and this may result in non-specific signal. If necessary, perform an additional blocking treatment with Levamisol (for ALP) or with 0.3 % solution of H2O2 in methanol (for peroxidase). Solutions ? Blocking solutions: commonly used blocking agents are BSA, non-fat dry milk, casein, gelatin. PBS containing 1 % Bovine Serum Albumin (BSA) ? Wash solution: usually PBS or Tris-buffered saline (pH 7.4) with detergent such as 0.05 % (v/v) Tween20 (TBST) ? Antibody solution: primary and secondary antibody should be diluted in 1x blocking solution to prevent non-specific binding. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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