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  1. Link

    adenovirus-type-5-antibody-ab6982.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus Adenovirus
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Anti-Adenovirus Type 5 antibody (ab6982)

  • Datasheet
  • SDS
Reviews (9)Q&A (20)References (71)

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Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)
  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
  • Functional Studies - Anti-Adenovirus Type 5 antibody (ab6982)
  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)

Key features and details

  • Rabbit polyclonal to Adenovirus Type 5
  • Suitable for: Neutralising, ELISA, WB, ICC/IF
  • Isotype: IgG

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Overview

  • Product name

    Anti-Adenovirus Type 5 antibody
  • Description

    Rabbit polyclonal to Adenovirus Type 5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Neutralising, ELISA, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Tissue, cells or virus corresponding to Adenovirus Type 5. Purified Adenovirus type 5

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Adenovirus

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising
Use at an assay dependent concentration.

This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137

ELISA (1)
Use at an assay dependent concentration.

Recognizes Ad5 immobilized on microtiter plates.

WB (5)
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration.
Notes
Neutralising
Use at an assay dependent concentration.

This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137

ELISA
Use at an assay dependent concentration.

Recognizes Ad5 immobilized on microtiter plates.

WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    Adenoviruses are DNA viruses that cause upper respiratory tract infections, conjunctivitis, and other infections in humans. Adenoviruses were discovered in 1953. About 47 different types have been identified since then, and about half of them are believed to cause human diseases. Infants and children are most commonly affected by adenoviruses. Adenovirus infections can occur throughout the year, but seem to be most common from fall to spring. Adenoviruses are responsible for 3-5% of acute respiratory infections in children and 2% of respiratory illnesses in adults.
  • Alternative names

    • Adenovirus 5 antibody
    • AdV 5 antibody

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)
    Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.
    ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.

    See Abreview

  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
    Western blot - Anti-Adenovirus Type 5 antibody (ab6982)

    Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).

    Bottom blot (B): As above, except ab6982 omitted.

    Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.
  • Functional Studies - Anti-Adenovirus Type 5 antibody (ab6982)
    Functional Studies - Anti-Adenovirus Type 5 antibody (ab6982)
    Additional information regarding Adenovirus type 5 capsid proteins. 
  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
    Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview
    All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    All lanes : human hepatocytes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 130 kDa why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocked with 5% milk

    See Abreview

  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
    Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.
    All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    Lane 1 : Whole cell lysate prepared from U2OS cells.
    Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit conjugated to IRDye 700DX


    Blocking step was performed using 5% milk for 1 hour at 23°C.

    See Abreview

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (71)

Publishing research using ab6982? Please let us know so that we can cite the reference in this datasheet.

ab6982 has been referenced in 71 publications.

  • Dalidowska I  et al. Heat Shock Protein 90 Chaperones E1A Early Protein of Adenovirus 5 and Is Essential for Replication of the Virus. Int J Mol Sci 22:N/A (2021). PubMed: 33670684
  • Saha B & Parks RJ Identification of human adenovirus replication inhibitors from a library of small molecules targeting cellular epigenetic regulators. Virology 555:102-110 (2021). PubMed: 33032802
  • Ho CT  et al. Combination of Mesenchymal Stem Cell-Delivered Oncolytic Virus with Prodrug Activation Increases Efficacy and Safety of Colorectal Cancer Therapy. Biomedicines 9:N/A (2021). PubMed: 34068264
  • Poulin KL  et al. Fusion of Large Polypeptides to Human Adenovirus Type 5 Capsid Protein IX Can Compromise Virion Stability and DNA Packaging Capacity. J Virol 94:N/A (2020). PubMed: 32522855
  • Wang Z  et al. Enhancing the antitumor activity of an engineered TRAIL-coated oncolytic adenovirus for treating acute myeloid leukemia. Signal Transduct Target Ther 5:40 (2020). PubMed: 32327638
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-9 of 9 Abreviews

Western blot abreview for Anti-Adenovirus Type 5 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
infected with Adv-iNOS
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

Submitted Mar 31 2017

Immunohistochemistry (Frozen sections) abreview for Anti-Adenovirus Type 5 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (AdV-infected submandibular salivary gland)
Specification
AdV-infected submandibular salivary gland
Fixative
Paraformaldehyde
Permeabilization
Yes - tritonX 0.2%
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 15°C
Read More

Abcam user community

Verified customer

Submitted Oct 02 2012

Western blot abreview for Anti-Adenovirus Type 5 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hek293)
Loading amount
25 µg
Specification
Hek293
Treatment
AdV-infected
Gel Running Conditions
Non-reduced Denaturing (4-12% Tris-Bis)
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Oct 02 2012

Immunohistochemistry (Frozen sections) abreview for Anti-Adenovirus Type 5 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 15°C
Read More

Abcam user community

Verified customer

Submitted Aug 24 2012

ELISA abreview for Anti-Adenovirus Type 5 antibody

Excellent
Abreviews
Abreviews
Application
ELISA
Sample
Human Cell (293e4)
Specification
293e4
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Type
Indirect
Read More

Abcam user community

Verified customer

Submitted Jan 27 2011

Western blot abreview for Anti-Adenovirus Type 5 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
35 µg
Specification
U2OS
Treatment
infected with Adenovirus 5
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

Submitted Jan 27 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-Adenovirus Type 5 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Specification
HeLa Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Oct 21 2010

Western blot abreview for Anti-Adenovirus Type 5 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (A293 Cells)
Loading amount
300 µg
Specification
A293 Cells
Gel Running Conditions
Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 01 2008

Western blot abreview for Anti-Adenovirus Type 5 antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
Read More

Mr. Oliver Scheibe

Verified customer

Submitted Feb 24 2006

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