Anti-Adenovirus Type 5 antibody (ab6982)
Key features and details
- Rabbit polyclonal to Adenovirus Type 5
- Suitable for: Neutralising, ELISA, WB, ICC/IF
- Isotype: IgG
Overview
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Product name
Anti-Adenovirus Type 5 antibody -
Description
Rabbit polyclonal to Adenovirus Type 5 -
Host species
Rabbit -
Tested applications
Suitable for: Neutralising, ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Tissue, cells or virus corresponding to Adenovirus Type 5. Purified Adenovirus type 5
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Neutralising |
Use at an assay dependent concentration.
This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137 |
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| ELISA | (1) |
Use at an assay dependent concentration.
Recognizes Ad5 immobilized on microtiter plates. |
| WB | (5) |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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Neutralising
Use at an assay dependent concentration. This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137 |
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ELISA
Use at an assay dependent concentration. Recognizes Ad5 immobilized on microtiter plates. |
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WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
Adenoviruses are DNA viruses that cause upper respiratory tract infections, conjunctivitis, and other infections in humans. Adenoviruses were discovered in 1953. About 47 different types have been identified since then, and about half of them are believed to cause human diseases. Infants and children are most commonly affected by adenoviruses. Adenovirus infections can occur throughout the year, but seem to be most common from fall to spring. Adenoviruses are responsible for 3-5% of acute respiratory infections in children and 2% of respiratory illnesses in adults. -
Alternative names
- Adenovirus 5 antibody
- AdV 5 antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.
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Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).
Bottom blot (B): As above, except ab6982 omitted.
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted. -
Functional Studies - Anti-Adenovirus Type 5 antibody (ab6982)Additional information regarding Adenovirus type 5 capsid proteins. -
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous AbreviewAll lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
All lanes : human hepatocytes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocked with 5% milk
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Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1 : Whole cell lysate prepared from U2OS cells.
Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-rabbit conjugated to IRDye 700DX
Blocking step was performed using 5% milk for 1 hour at 23°C.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (71)
ab6982 has been referenced in 71 publications.
- Dalidowska I et al. Heat Shock Protein 90 Chaperones E1A Early Protein of Adenovirus 5 and Is Essential for Replication of the Virus. Int J Mol Sci 22:N/A (2021). PubMed: 33670684
- Saha B & Parks RJ Identification of human adenovirus replication inhibitors from a library of small molecules targeting cellular epigenetic regulators. Virology 555:102-110 (2021). PubMed: 33032802
- Ho CT et al. Combination of Mesenchymal Stem Cell-Delivered Oncolytic Virus with Prodrug Activation Increases Efficacy and Safety of Colorectal Cancer Therapy. Biomedicines 9:N/A (2021). PubMed: 34068264
- Poulin KL et al. Fusion of Large Polypeptides to Human Adenovirus Type 5 Capsid Protein IX Can Compromise Virion Stability and DNA Packaging Capacity. J Virol 94:N/A (2020). PubMed: 32522855
- Wang Z et al. Enhancing the antitumor activity of an engineered TRAIL-coated oncolytic adenovirus for treating acute myeloid leukemia. Signal Transduct Target Ther 5:40 (2020). PubMed: 32327638