Key features and details
- Rabbit polyclonal to Adenovirus Type 5 E1A
- Suitable for: IHC-P, WB
- Isotype: IgG
Product nameAnti-Adenovirus Type 5 E1A antibody
DescriptionRabbit polyclonal to Adenovirus Type 5 E1A
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Other species
- Rat brain lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab204123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.
When using a fluorescent probe the recommended dilution range is 1/50 - 1/200
|WB||1/100 - 1/1000.|
RelevanceThe early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
- SwissProt: P03255 Other species
- Hadv 5 E1A antibody
- HAdv5 E1A antibody
All lanes : Anti-Adenovirus Type 5 E1A antibody (ab204123) at 1/200 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat liver lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Observed band size: 39 kDa why is the actual band size different from the predicted?
ab204123 has not yet been referenced specifically in any publications.