Product nameAnti-Adenylate cyclase 1/AC1 antibody
See all Adenylate cyclase 1/AC1 primary antibodies
DescriptionRabbit polyclonal to Adenylate cyclase 1/AC1
Detects endogenous levels of total Adenylate cyclase 1/AC1 protein.
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Adenylate cyclase 1/AC1 (internal sequence).
- Extracts from COLO205 cells
This product was previously labelled as Adenylate cyclase 1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab69597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 124 kDa).|
FunctionThis is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning.
Tissue specificityBrain, retina and adrenal medulla.
Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 2 guanylate cyclase domains.
- Information by UniProt
- Adenylate cyclase 1, fetal brain, type I antibody
- 3',5'-cyclic AMP synthetase antibody
- AC1 antibody
All lanes : Anti-Adenylate cyclase 1/AC1 antibody (ab69597) at 1/500 dilution
Lane 1 : Extracts from COLO205
Lane 2 : Extracts from COLO205 with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 124 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 150 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
IHC image of ab69597 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69597, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.