Adenylate Kinase Assay Kit (Cytotoxicity) (ab228557)

Overview

  • Product name

    Adenylate Kinase Assay Kit (Cytotoxicity)
  • Detection method

    Luminescent
  • Sample type

    Cell culture media
  • Assay type

    Enzyme activity (quantitative)
  • Product overview

    Adenylate Kinase Assay Kit (Cytotoxicity) (ab228557) measures the activity of the adenylate kinase released by damaged cells in a simple one-step procedure involving two chemical reactions.


    In the first reaction, adenylate kinase converts ADP to ATP. In the second reaction, luciferase catalyzes the formation of light from ATP and luciferin; the light is then measured using a luminometer or beta counter.


    The assay is highly sensitive and can be fully automated for high throughput applications.


    Review our cell health assay guide to learn about our kits to perform a cell viability assaycytotoxicity assay or cell proliferation assay.

  • Notes

    Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Adenylate kinase (AK) is a ubiquitous protein present in all eukaryotic and prokaryotic cells and is rapidly released into culture medium upon damage to the plasma membrane.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 500 tests
    AK Assay Buffer 1 x 50ml
    AK Detection Reagent Lyophilized 5 vials
  • Alternative names

    • adk

Protocols

References

ab228557 has not yet been referenced specifically in any publications.

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