Product nameAdenylate Kinase Assay Kit (Cytotoxicity)
Sample typeCell culture media
Assay typeEnzyme activity (quantitative)
Adenylate Kinase Assay Kit (Cytotoxicity) (ab228557) measures the activity of the adenylate kinase released by damaged cells in a simple one-step procedure involving two chemical reactions.
In the first reaction, adenylate kinase converts ADP to ATP. In the second reaction, luciferase catalyzes the formation of light from ATP and luciferin; the light is then measured using a luminometer or beta counter.
The assay is highly sensitive and can be fully automated for high throughput applications.
Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Adenylate kinase (AK) is a ubiquitous protein present in all eukaryotic and prokaryotic cells and is rapidly released into culture medium upon damage to the plasma membrane.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 500 tests AK Assay Buffer 1 x 50ml AK Detection Reagent Lyophilized 5 vials
ab228557 has not yet been referenced specifically in any publications.