Anti-ADFP antibody (ab230773)
Key features and details
- Rabbit polyclonal to ADFP
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADFP antibody
See all ADFP primary antibodies -
Description
Rabbit polyclonal to ADFP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide corresponding to Human ADFP aa 186-214 conjugated to keyhole limpet haemocyanin.
Sequence:LVEQYLPLTEEELEKEAKKVEGFDLVQKP
Database link: Q99541 -
Positive control
- IHC-P: Human small intestine and liver tissue. WB: Jurkat cells lysate. Flow: Jurkat cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab230773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 48 kDa. | |
| Flow Cyt | 1/10 - 1/50. |
Target
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Function
May be involved in development and maintenance of adipose tissue. -
Tissue specificity
Milk lipid globules. -
Sequence similarities
Belongs to the perilipin family. -
Post-translational
modificationsAcylated; primarily with C14, C16 and C18 fatty acids. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 123 Human
- Omim: 103195 Human
- SwissProt: Q99541 Human
- SwissProt: Q4PLW0 Pig
- Unigene: 3416 Human
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Alternative names
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
Images
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Western blot - Anti-ADFP antibody (ab230773)Anti-ADFP antibody (ab230773) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Developed using the ECL technique.
Predicted band size: 48 kDa -
Flow Cytometry - Anti-ADFP antibody (ab230773)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labelling ADFP using ab230773 at 1/10 dilution (green) compared to a negative control cell (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP antibody (ab230773)Formalin-fixed, paraffin-embedded human liver tissue stained for ADFP using ab230773 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP antibody (ab230773)Formalin-fixed, paraffin-embedded human small intestine tissue stained for ADFP using ab230773 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab230773 has not yet been referenced specifically in any publications.
