Key features and details
- Rabbit polyclonal to ADFP
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADFP antibody
See all ADFP primary antibodies
DescriptionRabbit polyclonal to ADFP
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Pig
- IHC-P: Human small intestine and liver tissue. WB: Jurkat cells lysate. Flow: Jurkat cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab230773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 48 kDa.|
|Flow Cyt||1/10 - 1/50.|
FunctionMay be involved in development and maintenance of adipose tissue.
Tissue specificityMilk lipid globules.
Sequence similaritiesBelongs to the perilipin family.
modificationsAcylated; primarily with C14, C16 and C18 fatty acids.
- Information by UniProt
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
Anti-ADFP antibody (ab230773) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Developed using the ECL technique.
Predicted band size: 48 kDa
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labelling ADFP using ab230773 at 1/10 dilution (green) compared to a negative control cell (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed, paraffin-embedded human liver tissue stained for ADFP using ab230773 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human small intestine tissue stained for ADFP using ab230773 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230773 has not yet been referenced specifically in any publications.