Anti-ADFP antibody (ab230773)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ADFP antibody
See all ADFP primary antibodies -
Description
Rabbit polyclonal to ADFP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide corresponding to Human ADFP aa 186-214 conjugated to keyhole limpet haemocyanin.
Sequence:LVEQYLPLTEEELEKEAKKVEGFDLVQKP
Database link: Q99541 -
Positive control
- IHC-P: Human small intestine and liver tissue. WB: Jurkat cells lysate. Flow: Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab230773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 48 kDa. | |
| Flow Cyt | 1/10 - 1/50. |
Target
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Function
May be involved in development and maintenance of adipose tissue. -
Tissue specificity
Milk lipid globules. -
Sequence similarities
Belongs to the perilipin family. -
Post-translational
modificationsAcylated; primarily with C14, C16 and C18 fatty acids. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 123 Human
- Omim: 103195 Human
- SwissProt: Q99541 Human
- SwissProt: Q4PLW0 Pig
- Unigene: 3416 Human
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Alternative names
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
Images
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Western blot - Anti-ADFP antibody (ab230773)Anti-ADFP antibody (ab230773) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Developed using the ECL technique.
Predicted band size: 48 kDa -
Flow Cytometry - Anti-ADFP antibody (ab230773)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labelling ADFP using ab230773 at 1/10 dilution (green) compared to a negative control cell (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP antibody (ab230773)Formalin-fixed, paraffin-embedded human liver tissue stained for ADFP using ab230773 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP antibody (ab230773)Formalin-fixed, paraffin-embedded human small intestine tissue stained for ADFP using ab230773 at 1/100 dilution in immunohistochemical analysis.
Datasheets and documents
References
ab230773 has not yet been referenced specifically in any publications.
