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  1. Link

    adfp-antibody-ab52356.pdf

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Cardiovascular Hypoxia Hypoxia-Regulated
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Anti-ADFP antibody (ab52356)

  • Datasheet
Reviews (3)Q&A (1)References (25)

Product price, shipping and contact information

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Abpromise

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Find out more.

Western blot - Anti-ADFP antibody (ab52356)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP antibody (ab52356)
  • Immunocytochemistry - Anti-ADFP antibody (ab52356)

Key features and details

  • Rabbit polyclonal to ADFP
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-ADFP antibody [EPR3713] (ab108323)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-ADFP antibody
    See all ADFP primary antibodies
  • Description

    Rabbit polyclonal to ADFP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide made to a C-terminal region within residues 350-425 of the mouse ADFP protein.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituents: 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Hypoxia
    • Hypoxia-Regulated
    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Lipid Droplet Protein
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipid transport
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB (2)
Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/200.
Notes
ICC
Use at an assay dependent concentration.
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/200.

Target

  • Function

    May be involved in development and maintenance of adipose tissue.
  • Tissue specificity

    Milk lipid globules.
  • Sequence similarities

    Belongs to the perilipin family.
  • Post-translational
    modifications

    Acylated; primarily with C14, C16 and C18 fatty acids.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q99541 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 123 Human
    • Entrez Gene: 11520 Mouse
    • Entrez Gene: 298199 Rat
    • Omim: 103195 Human
    • SwissProt: Q99541 Human
    • SwissProt: P43883 Mouse
    • Unigene: 3416 Human
    • Unigene: 381 Mouse
    • Alternative names

      • ADFP antibody
      • Adipophilin antibody
      • Adipose differentiation related protein antibody
      • Adipose differentiation-related protein antibody
      • ADRP antibody
      • MGC10598 antibody
      • Perilipin-2 antibody
      • PLIN2 antibody
      • PLIN2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-ADFP antibody (ab52356)
      Western blot - Anti-ADFP antibody (ab52356)
      Anti-ADFP antibody (ab52356) at 2 µg/ml + mouse liver lysate

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP antibody (ab52356)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP antibody (ab52356)

      Immunohistochemical analysis of mouse liver tissue staining ADFP using ab52356. 

    • Immunocytochemistry - Anti-ADFP antibody (ab52356)
      Immunocytochemistry - Anti-ADFP antibody (ab52356)

      Ab52356 staining ADFP in HeLa cells at 1/50 dilution. Hoechst 33258 was used as a nuclear counterstain. 

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (25)

    Publishing research using ab52356? Please let us know so that we can cite the reference in this datasheet.

    ab52356 has been referenced in 25 publications.

    • Schöbel A  et al. Hepatitis C virus infection restricts human LINE-1 retrotransposition in hepatoma cells. PLoS Pathog 17:e1009496 (2021). PubMed: 33872335
    • Lu YS  et al. UVA Induced Oxidative Stress Was Inhibited by Paeoniflorin/Nrf2 Signaling or PLIN2. Front Pharmacol 11:736 (2020). PubMed: 32499710
    • Zhou C  et al. Gadofullerene inhibits the degradation of apolipoprotein B100 and boosts triglyceride transport for reversing hepatic steatosis. Sci Adv 6:N/A (2020). PubMed: 32917715
    • Li R  et al. Myofibroblast contraction is essential for generating and regenerating the gas-exchange surface. J Clin Invest 130:2859-2871 (2020). PubMed: 32338642
    • Hagan AS  et al. Identification of a FGF18-expressing alveolar myofibroblast that is developmentally cleared during alveologenesis. Development 147:N/A (2020). PubMed: 31862844
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-ADFP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Lung)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Lung
    Blocking step
    1% BSA and 1.5% Donkey Serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 11 2018

    Western blot abreview for Anti-ADFP antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse muscle)
    Loading amount
    40 µg
    Specification
    Mouse muscle
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 25 2011

    Western blot abreview for Anti-ADFP antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (AML-12 cell(liver cell line))
    Loading amount
    30 µg
    Specification
    AML-12 cell(liver cell line)
    Treatment
    2uM Rosiglitazone for 24hrs
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 30 2009

    Question

    ab52356: IHC-Fr, IHC-P (untested) from mouse
    first lot worked very nicely
    second lot (GR49906-1) did work in ICC/IF with mouse primary cells, but neither in IHC-Fr nor IHC-P
    Ab: 1/50, 1/100, 1/200 overnight 4 degree
    block with BSA+goat serum, goat serum, or commercial blocking reagent
    AR: citrate, proteinase K, trypsin or combination
    detection: Alexa 488 or DAB (working otherwise)

    ab23194: will test in mouse

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2012

    Answer

    Thank you for your phone call.

    The free of charge replacement information for ab52356 can be found below. I am also sending youthe testing discount code for trying ab23194 in mouse - at the bottom of the email.


    I am sorry to hear that our product ab52356 did not perform as before and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx.

    To check the status of the order please contact our Customer Service team and reference this number.

    Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    ====================================================

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab23194 in mouse. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Mar 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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