Anti-ADFP antibody (ab52356)

Reviews (3)Q&A (1)References (17)

Key features and details

  • Rabbit polyclonal to ADFP
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ADFP antibody
    See all ADFP primary antibodies

  • Description

    Rabbit polyclonal to ADFP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    A synthetic peptide made to a C-terminal region within residues 350-425 of the mouse ADFP protein.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab52356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/200.

Target

Images

  • Western blot - Anti-ADFP antibody (ab52356)
    Western blot - Anti-ADFP antibody (ab52356)
    Anti-ADFP antibody (ab52356) at 2 µg/ml + mouse liver lysate

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP antibody (ab52356)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP antibody (ab52356)

    Immunohistochemical analysis of mouse liver tissue staining ADFP using ab52356. 

  • Immunocytochemistry - Anti-ADFP antibody (ab52356)
    Immunocytochemistry - Anti-ADFP antibody (ab52356)

    Ab52356 staining ADFP in HeLa cells at 1/50 dilution. Hoechst 33258 was used as a nuclear counterstain. 

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (17)

Publishing research using ab52356? Please let us know so that we can cite the reference in this datasheet.

ab52356 has been referenced in 17 publications.

  • Lu YS  et al. UVA Induced Oxidative Stress Was Inhibited by Paeoniflorin/Nrf2 Signaling or PLIN2. Front Pharmacol 11:736 (2020). PubMed: 32499710
  • Laufman O  et al. Viral Generated Inter-Organelle Contacts Redirect Lipid Flux for Genome Replication. Cell 178:275-289.e16 (2019). PubMed: 31204099
  • Chen X  et al. Suppressed hepatocyte proliferation via a ROS-HNE-P21 pathway is associated with nicotine- and cotinine-enhanced alcoholic fatty liver in mice. Biochem Biophys Res Commun 512:119-124 (2019). PubMed: 30876690
  • McCulley DJ  et al. PBX transcription factors drive pulmonary vascular adaptation to birth. J Clin Invest 128:655-667 (2018). PubMed: 29251627
  • Li R  et al. Pdgfra marks a cellular lineage with distinct contributions to myofibroblasts in lung maturation and injury response. Elife 7:N/A (2018). PubMed: 30178747
View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-ADFP antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lung)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Lung
Blocking step
1% BSA and 1.5% Donkey Serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 11 2018

Question

ab52356: IHC-Fr, IHC-P (untested) from mouse
first lot worked very nicely
second lot (GR49906-1) did work in ICC/IF with mouse primary cells, but neither in IHC-Fr nor IHC-P
Ab: 1/50, 1/100, 1/200 overnight 4 degree
block with BSA+goat serum, goat serum, or commercial blocking reagent
AR: citrate, proteinase K, trypsin or combination
detection: Alexa 488 or DAB (working otherwise)

ab23194: will test in mouse

Read More
Answer

Thank you for your phone call.

The free of charge replacement information for ab52356 can be found below. I am also sending youthe testing discount code for trying ab23194 in mouse - at the bottom of the email.


I am sorry to hear that our product ab52356 did not perform as before and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

====================================================

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab23194 in mouse. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I wish you the best of luck with your research.

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Western blot abreview for Anti-ADFP antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse muscle)
Loading amount
40 µg
Specification
Mouse muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 25 2011

Western blot abreview for Anti-ADFP antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (AML-12 cell(liver cell line))
Loading amount
30 µg
Specification
AML-12 cell(liver cell line)
Treatment
2uM Rosiglitazone for 24hrs
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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