Overview

  • Product name

  • Description

    Rabbit polyclonal to ADFP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human, Rhesus monkey
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide made to a C-terminal region within residues 350-425 of the mouse ADFP protein.

  • Positive control

    • Mouse and human liver lysate

Applications

Our Abpromise guarantee covers the use of ab52356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20335253
WB Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/200.

Target

Images

  • Anti-ADFP antibody (ab52356) at 2 µg/ml + mouse liver lysate

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunohistochemical analysis of mouse liver tissue staining ADFP using ab52356. 

  • Ab52356 staining ADFP in HeLa cells at 1/50 dilution. Hoechst 33258 was used as a nuclear counterstain. 

References

This product has been referenced in:

  • Chen X  et al. Suppressed hepatocyte proliferation via a ROS-HNE-P21 pathway is associated with nicotine- and cotinine-enhanced alcoholic fatty liver in mice. Biochem Biophys Res Commun 512:119-124 (2019). Read more (PubMed: 30876690) »
  • McCulley DJ  et al. PBX transcription factors drive pulmonary vascular adaptation to birth. J Clin Invest 128:655-667 (2018). Read more (PubMed: 29251627) »
See all 14 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lung)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Lung
Blocking step
1% BSA and 1.5% Donkey Serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 11 2018

Answer

Thank you for your phone call.

The free of charge replacement information for ab52356 can be found below. I am also sending youthe testing discount code for trying ab23194 in mouse - at the bottom of the email.


I am sorry to hear that our product ab52356 did not perform as before and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

====================================================

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab23194 in mouse. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I wish you the best of luck with your research.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse muscle)
Loading amount
40 µg
Specification
Mouse muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 25 2011

Application
Western blot
Sample
Mouse Cell lysate - whole cell (AML-12 cell(liver cell line))
Loading amount
30 µg
Specification
AML-12 cell(liver cell line)
Treatment
2uM Rosiglitazone for 24hrs
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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