Anti-ADFP antibody (ab52356)
Key features and details
- Rabbit polyclonal to ADFP
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ADFP antibody
See all ADFP primary antibodies -
Description
Rabbit polyclonal to ADFP -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
A synthetic peptide made to a C-terminal region within residues 350-425 of the mouse ADFP protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: 30% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB | (2) |
Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
1/200.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/200. |
Target
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Function
May be involved in development and maintenance of adipose tissue. -
Tissue specificity
Milk lipid globules. -
Sequence similarities
Belongs to the perilipin family. -
Post-translational
modificationsAcylated; primarily with C14, C16 and C18 fatty acids. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 123 Human
- Entrez Gene: 11520 Mouse
- Entrez Gene: 298199 Rat
- Omim: 103195 Human
- SwissProt: Q99541 Human
- SwissProt: P43883 Mouse
- Unigene: 3416 Human
- Unigene: 381 Mouse
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Alternative names
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
Images
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Anti-ADFP antibody (ab52356) at 2 µg/ml + mouse liver lysate
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunohistochemical analysis of mouse liver tissue staining ADFP using ab52356.
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Ab52356 staining ADFP in HeLa cells at 1/50 dilution. Hoechst 33258 was used as a nuclear counterstain.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (25)
ab52356 has been referenced in 25 publications.
- Schöbel A et al. Hepatitis C virus infection restricts human LINE-1 retrotransposition in hepatoma cells. PLoS Pathog 17:e1009496 (2021). PubMed: 33872335
- Lu YS et al. UVA Induced Oxidative Stress Was Inhibited by Paeoniflorin/Nrf2 Signaling or PLIN2. Front Pharmacol 11:736 (2020). PubMed: 32499710
- Zhou C et al. Gadofullerene inhibits the degradation of apolipoprotein B100 and boosts triglyceride transport for reversing hepatic steatosis. Sci Adv 6:N/A (2020). PubMed: 32917715
- Li R et al. Myofibroblast contraction is essential for generating and regenerating the gas-exchange surface. J Clin Invest 130:2859-2871 (2020). PubMed: 32338642
- Hagan AS et al. Identification of a FGF18-expressing alveolar myofibroblast that is developmentally cleared during alveologenesis. Development 147:N/A (2020). PubMed: 31862844