Overview

  • Product name

  • Description

    Rabbit polyclonal to ADFP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ADFP aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90212)

  • Positive control

    • Ab78920 gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293. ICC/IF: HepG2 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab78920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

Images

  • All lanes : Anti-ADFP antibody (ab78920) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa
    Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • ab78920 staining ADFP in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody ab78920 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • Anti-ADFP antibody (ab78920) at 1 µg/ml + Recombinant Human ADFP protein (ab73631) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa


    Exposure time: 1 minute

References

This product has been referenced in:

  • Yeshaw WM  et al. Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility. Elife 8:N/A (2019). WB . Read more (PubMed: 30741634) »
  • Chen H  et al. Lipophagy contributes to long-term storage of spermatozoa in the epididymis of the Chinese soft-shelled turtle Pelodiscus sinensis. Reprod Fertil Dev N/A:N/A (2018). Read more (PubMed: 30526797) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Sample
African Green Monkey Cell (Rhesus monkey kidney cells)
Specification
Rhesus monkey kidney cells
Permeabilization
Yes - 0.2% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 13 2014

Application
Western blot
Sample
Human Cell lysate - whole cell (Huh7)
Loading amount
50 µg
Specification
Huh7
Treatment
siADRP 10uM
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Mrs. Alina Macovei

Verified customer

Submitted Feb 21 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepaRG)
Specification
HepaRG
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Mrs. Alina Macovei

Verified customer

Submitted Jun 19 2012

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