Overview

  • Product name

    Anti-ADFP antibody [ADFP/1366]
    See all ADFP primary antibodies
  • Description

    Mouse monoclonal [ADFP/1366] to ADFP
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ADFP aa 249-376. The exact sequence is proprietary.
    Sequence:

    TI SQLHSTVHLI EFARKNVYSA NQKIQDAQDK LYLSWVEWKR SIGYDDTDES HCAEHIESRT LAIARNLTQQ LQTTCHTLLS NIQGVPQNIQ DQAKHMGVMA GDIYSVFRNA ASFKEVSDSL LTSSKG


    Database link: Q99541

  • Positive control

    • Human adrenal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab219299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in development and maintenance of adipose tissue.
  • Tissue specificity

    Milk lipid globules.
  • Sequence similarities

    Belongs to the perilipin family.
  • Post-translational
    modifications

    Acylated; primarily with C14, C16 and C18 fatty acids.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADFP antibody
    • Adipophilin antibody
    • Adipose differentiation related protein antibody
    • Adipose differentiation-related protein antibody
    • ADRP antibody
    • MGC10598 antibody
    • Perilipin-2 antibody
    • PLIN2 antibody
    • PLIN2_HUMAN antibody
    see all

Images

  • ab219299 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Immunohistochemical analysis of paraffin embedded, formalin fixed Human adrenal tissue labeling ADFP using ab219299 at 1 µg/ml

References

ab219299 has not yet been referenced specifically in any publications.

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