Recombinant
RabMAb

Recombinant Anti-ADFP antibody [EPR3713] (ab108323)

Rabbit recombinant monoclonal ADFP antibody [EPR3713]. Validated in WB, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 7 publication(s). Independently reviewed in 2 review(s).

Overview

  • Product name

    Anti-ADFP antibody [EPR3713]
    See all ADFP primary antibodies
  • Description

    Rabbit monoclonal [EPR3713] to ADFP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-Fr or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ADFP aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Fetal liver, HepG2, JAR, Human milk, Mouse brain and Rat brain lysates; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/500.
  • Application notes
    Is unsuitable for IHC-Fr or IP.
  • Target

    Images

    • All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : JAR lysate
      Lane 4 : Human milk lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 48 kDa

    • Ab108323 staining ADFP in HepG2 (human hepatocellular carcinoma epithelial cell) cells by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% TritonX-100. Samples were incubated with primary antibody at 1/500 dilution (2.5µg/ml). An Alexa Fluor® 488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2µg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain antibody at 1/200 dilution (2.5µg/ml). DAPI was used as the nuclear counterstain. Confocal image showing cytoplasmic staining in HepG2 cells. 

    • Overlay histogram showing Jurkat cells stained with ab108323 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108323, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 48 kDa

    References

    This product has been referenced in:

    • Presa N  et al. Vitamin E alleviates non-alcoholic fatty liver disease in phosphatidylethanolamine N-methyltransferase deficient mice. Biochim Biophys Acta Mol Basis Dis 1865:14-25 (2019). Read more (PubMed: 30300671) »
    • Bai Y  et al. Lipid storage and lipophagy regulates ferroptosis. Biochem Biophys Res Commun 508:997-1003 (2019). Read more (PubMed: 30545638) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    50 µg
    Treatment
    Western diet 12 weeks
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Sep 10 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (breast)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Specification
    breast
    Blocking step
    Ventana antibody dilution buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 35°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted May 14 2015

    Question
    Answer

    Thank you for your call today and for letting us know about the trouble with ab108323.

    Please keep me updated about any new results or progress after trying the alterations that we discussed. We do fully guarantee this antibody to work in your assay, so I would be happy to send a replacement if needed.

    I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

    Read More

    Answer

    Thank you for your inquiry.


    The immunogenpeptide for ab108323maps between amino acids 1 - 70 of human ADFP (UniProt: Q99541). We cannot guarantee that this antibody will work in an ELISA as this is untested, but theoretically if you find an antibody that maps to a different epitope it may work in sELISA with ab108323. Again, none of our other anti-ADFP antibodies are tested in ELISA, but ab37516 maps to a.a. 71 - 240, ab52355 maps to a.a. 150 - 250, and ab52356 maps to a.a. 350 - 425. Since these are adifferent epitope,they may work in sELISA with ab108323.


    However, since these are not tested together in sELISA, we wouldn't be able to guarantee it. If you would like to test these antibodies together in sELISA, you would qualify for our 100% testing discount program. Essentially you would buy both antibodies up-front, test them together in sELISA, submit 2 Abreviews (1 for each product) about the protocol tested, and even if they don't work, you would earn 2 discount codes worth 2 free primary antibodies to use within 4 months.


    Please let me know if this is something you would be interested in. I hope this information helps.

    Read More

    Answer

    Thank you for your patience.

    I have contacted the lab and, unfortunately, they confirmed that this antibody was tested for IHC and IP, but IHC staining was non-specific and IP results were negative.

    I hope this information is nonetheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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