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Rabbit polyclonal to ADH1B
HumanPredicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Chimpanzee, Drosophila melanogaster, Zebrafish
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (STAGKVIKCK AAVLWEVKKP FSIEDVEVAP PKAYEVRIKM VAVGICHTDD) of Human ADH1B (NP_000659).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
Belongs to the zinc-containing alcohol dehydrogenase family.
Information by UniProt
- ADH beta subunit antibody
- ADH1B antibody
- ADH1B_HUMAN antibody
Western blot - Anti-ADH1B antibody (ab83475)
Anti-ADH1B antibody (ab83475) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
40 kDaObserved band size:
44 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"