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    adh5-antibody-epr12885b-ab177932.pdf

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Signal Transduction Metabolism Alcohol metabolism
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RecombinantRabMAb

Recombinant Anti-ADH5 antibody [EPR12885(B)] (ab177932)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (3)

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Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
  • Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12885(B)] to ADH5
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Assay
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Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
Protein
Product image
Recombinant Human ADH5 protein (ab124573)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-ADH5 antibody [EPR12885(B)]
    See all ADH5 primary antibodies
  • Description

    Rabbit monoclonal [EPR12885(B)] to ADH5
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ADH5 aa 1-100. The exact sequence is proprietary.
    Database link: P11766

  • Positive control

    • WB: Human liver, Mouse liver, Human fetal liver, HepG2, Human fetal kidney, SH-SY5Y, Mouse brain, Rat liver, and Rat brain lysates. IHC-P: human kidney tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12885(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism

Associated products

  • Alternative Versions

    • Anti-ADH5 antibody [EPR12885(B)] - BSA and Azide free (ab250008)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
  • Recombinant Protein

    • Recombinant Human ADH5 protein (ab124573)
  • Related Products

    • Recombinant Human ADH5 protein (ab124573)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

For unpurified use at 1/50-1/100

Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

For unpurified use at 1/50-1/100

Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
  • Sequence similarities

    Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P11766 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 128 Human
    • Entrez Gene: 11532 Mouse
    • Entrez Gene: 100145871 Rat
    • Omim: 103710 Human
    • SwissProt: P11766 Human
    • SwissProt: P28474 Mouse
    • SwissProt: P12711 Rat
    • Unigene: 78989 Human
    • Unigene: 3874 Mouse
    • Unigene: 222115 Rat
    see all
  • Alternative names

    • ADH 3 antibody
    • ADH5 antibody
    • ADHX antibody
    • ADHX_HUMAN antibody
    • Alcohol dehydrogenase (class III) chi polypeptide antibody
    • alcohol dehydrogenase 5 (class III) chi polypeptide antibody
    • Alcohol dehydrogenase 5 antibody
    • Alcohol dehydrogenase class 3 antibody
    • Alcohol dehydrogenase class chi chain antibody
    • Alcohol dehydrogenase class III antibody
    • Alcohol dehydrogenase class-3 antibody
    • Alcohol dehydrogenase class-III antibody
    • class III alcohol dehydrogenase 5 chi subunit antibody
    • FALDH antibody
    • FDH antibody
    • formaldehyde dehydrogenase antibody
    • Glutathione dependent formaldehyde dehydrogenase antibody
    • Glutathione-dependent formaldehyde dehydrogenase antibody
    • GSH-FDH antibody
    • GSNOR antibody
    • hydroxymethyllutathione dehydrogenase antibody
    • S-(hydroxymethyl)glutathione dehydrogenase antibody
    see all

Images

  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified) + Human liver lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling ADH5 with purified ab177932 at 1/8000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified)

    Lane 1 : Mouse liver lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat liver lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    ADH5 is highly expressed in liver, kidney and low expressed in brain as is described in PMID: 26412304

  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/1000 dilution ((unpurified))

    Lane 1 : Human fetal liver lysates
    Lane 2 : HepG2 lysates
    Lane 3 : Human fetal kidney lysates
    Lane 4 : SH-SY5Y lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-ADH5 antibody [EPR12885(B)] (ab177932)
    Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab177932? Please let us know so that we can cite the reference in this datasheet.

ab177932 has been referenced in 3 publications.

  • Chen G  et al. bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway. Redox Biol 41:101904 (2021). PubMed: 33706169
  • Pan L  et al. S-Nitrosylation of Plastin-3 Exacerbates Thoracic Aortic Dissection Formation via Endothelial Barrier Dysfunction. Arterioscler Thromb Vasc Biol 40:175-188 (2020). PubMed: 31694393
  • Chen W  et al. Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. J Cell Mol Med 23:8161-8172 (2019). PubMed: 31565850

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunoprecipitation abreview for Anti-ADH5 antibody [EPR12885(B)]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (MEF cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
MEF cells
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 23 2018

Western blot abreview for Anti-ADH5 antibody [EPR12885(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 25 2018

Western blot abreview for Anti-ADH5 antibody [EPR12885(B)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
293T cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 03 2018

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