Recombinant
RabMAb

Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Abreviews (2) Submit a Question

Overview

  • Product name
    Anti-ADH5 antibody [EPR12885(B)]
    See all ADH5 primary antibodies
  • Description
    Rabbit monoclonal [EPR12885(B)] to ADH5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ADH5 aa 1-100. The exact sequence is proprietary.
    Database link: P11766

  • Positive control
    • Human fetal liver, HepG2, Human fetal kidney and SH-SY5Y lysates. Human brain and kidney tissue. Permeabilized SH-SY5Y cells. Immunoprecipitation pellet from Human fetal liver lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function
      Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
    • Sequence similarities
      Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • ADH 3 antibody
      • ADH5 antibody
      • ADHX antibody
      • ADHX_HUMAN antibody
      • Alcohol dehydrogenase (class III) chi polypeptide antibody
      • alcohol dehydrogenase 5 (class III) chi polypeptide antibody
      • Alcohol dehydrogenase 5 antibody
      • Alcohol dehydrogenase class 3 antibody
      • Alcohol dehydrogenase class chi chain antibody
      • Alcohol dehydrogenase class III antibody
      • Alcohol dehydrogenase class-3 antibody
      • Alcohol dehydrogenase class-III antibody
      • class III alcohol dehydrogenase 5 chi subunit antibody
      • FALDH antibody
      • FDH antibody
      • formaldehyde dehydrogenase antibody
      • Glutathione dependent formaldehyde dehydrogenase antibody
      • Glutathione-dependent formaldehyde dehydrogenase antibody
      • GSH-FDH antibody
      • GSNOR antibody
      • hydroxymethyllutathione dehydrogenase antibody
      • S-(hydroxymethyl)glutathione dehydrogenase antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADH5 with ab177932 at a 1/50 dilution.

    • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/1000 dilution

      Lane 1 : Human fetal liver lysates
      Lane 2 : HepG2 lysates
      Lane 3 : Human fetal kidney lysates
      Lane 4 : SH-SY5Y lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADH5 with ab177932 at a 1/50 dilution.

    • Flow Cytometry - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Flow Cytometry - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

      Flow cytometric analysis of permeabilized SH-SY5Y cells using ab177932 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Immunoprecipitation - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Immunoprecipitation - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

      Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver lysate or (Lane 2) 1XPBS (negative control) using ab177932 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab177932 has not yet been referenced specifically in any publications.

    Publishing research using ab177932? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    2 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-ADH5 antibody [EPR12885(B)]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    MEF cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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    Verified customer

    Submitted May 25 2018

    Western blot abreview for Anti-ADH5 antibody [EPR12885(B)]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    293T cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 03 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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