Recombinant
RabMAb

Recombinant Anti-ADH5 antibody [EPR12885(B)] (ab177932)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12885(B)] to ADH5
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ADH5 antibody [EPR12885(B)]
    See all ADH5 primary antibodies

  • Description

    Rabbit monoclonal [EPR12885(B)] to ADH5

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ADH5 aa 1-100. The exact sequence is proprietary.
    Database link: P11766

  • Positive control

    • WB: Human liver, Mouse liver, Human fetal liver, HepG2, Human fetal kidney, SH-SY5Y, Mouse brain, Rat liver, and Rat brain lysates. IHC-P: human kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab177932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
IHC-P 1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

For unpurified use at 1/50-1/100
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.

    • Sequence similarities

      Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ADH 3 antibody
      • ADH5 antibody
      • ADHX antibody
      • ADHX_HUMAN antibody
      • Alcohol dehydrogenase (class III) chi polypeptide antibody
      • alcohol dehydrogenase 5 (class III) chi polypeptide antibody
      • Alcohol dehydrogenase 5 antibody
      • Alcohol dehydrogenase class 3 antibody
      • Alcohol dehydrogenase class chi chain antibody
      • Alcohol dehydrogenase class III antibody
      • Alcohol dehydrogenase class-3 antibody
      • Alcohol dehydrogenase class-III antibody
      • class III alcohol dehydrogenase 5 chi subunit antibody
      • FALDH antibody
      • FDH antibody
      • formaldehyde dehydrogenase antibody
      • Glutathione dependent formaldehyde dehydrogenase antibody
      • Glutathione-dependent formaldehyde dehydrogenase antibody
      • GSH-FDH antibody
      • GSNOR antibody
      • hydroxymethyllutathione dehydrogenase antibody
      • S-(hydroxymethyl)glutathione dehydrogenase antibody
      see all

    Images

    • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified)

      Lane 1 : Mouse liver lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Rat liver lysates
      Lane 4 : Rat brain lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 40 kDa
      Observed band size: 40 kDa



      ADH5 is highly expressed in liver, kidney and low expressed in brain as is described in PMID: 26412304

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling ADH5 with purified ab177932 at 1/8000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified) + Human liver lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 40 kDa
      Observed band size: 40 kDa

    • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/1000 dilution ((unpurified))

      Lane 1 : Human fetal liver lysates
      Lane 2 : HepG2 lysates
      Lane 3 : Human fetal kidney lysates
      Lane 4 : SH-SY5Y lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    Publishing research using ab177932? Please let us know so that we can cite the reference in this datasheet.

    ab177932 has been referenced in 2 publications.

    • Pan L  et al. S-Nitrosylation of Plastin-3 Exacerbates Thoracic Aortic Dissection Formation via Endothelial Barrier Dysfunction. Arterioscler Thromb Vasc Biol 40:175-188 (2020). PubMed: 31694393
    • Chen W  et al. Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. J Cell Mol Med 23:8161-8172 (2019). PubMed: 31565850

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-ADH5 antibody [EPR12885(B)]

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (MEF cells)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    MEF cells
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 23 2018

    Western blot abreview for Anti-ADH5 antibody [EPR12885(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    MEF cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 25 2018

    Western blot abreview for Anti-ADH5 antibody [EPR12885(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    293T cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 03 2018

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