Recombinant Anti-ADH5 antibody [EPR12885(B)] (ab177932)
![Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)](https://www.abcam.com/ps/products/177/ab177932/Images/ab177932-365450-anti-adh5-antibody-epr12885-b-westernblot-brain-human.jpg "Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12885(B)] to ADH5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ADH5 antibody [EPR12885(B)]
See all ADH5 primary antibodies -
Description
Rabbit monoclonal [EPR12885(B)] to ADH5 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ADH5 aa 1-100. The exact sequence is proprietary.
Database link: P11766 -
Positive control
- WB: Human liver, Mouse liver, Human fetal liver, HepG2, Human fetal kidney, SH-SY5Y, Mouse brain, Rat liver, and Rat brain lysates. IHC-P: human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12885(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
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| IHC-P |
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/50-1/100 |
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa. |
|
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/50-1/100 |
Target
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Function
Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. -
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 128 Human
- Entrez Gene: 11532 Mouse
- Entrez Gene: 100145871 Rat
- Omim: 103710 Human
- SwissProt: P11766 Human
- SwissProt: P28474 Mouse
- SwissProt: P12711 Rat
- Unigene: 78989 Human
see all -
Alternative names
- ADH 3 antibody
- ADH5 antibody
- ADHX antibody
see all
Images
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Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified) + Human liver lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)](https://www.abcam.com/ps/products/177/ab177932/Images/ab177932-6-anti-adh5-antibody-epr12885-b-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling ADH5 with purified ab177932 at 1/8000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)](https://www.abcam.com/ps/products/177/ab177932/Images/ab177932-365451-anti-adh5-antibody-epr12885-b-westernblot-mouse-rat.jpg)
Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution (Purified)
Lane 1 : Mouse liver lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat liver lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaADH5 is highly expressed in liver, kidney and low expressed in brain as is described in PMID: 26412304
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![Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)](https://www.abcam.com/ps/products/177/ab177932/Images/ab177932-205899-anti-adh5-antibody-epr12885b-western-blot.jpg)
Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/1000 dilution ((unpurified))
Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 lysates
Lane 3 : Human fetal kidney lysates
Lane 4 : SH-SY5Y lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)](https://www.abcam.com/ps/products/177/ab177932/Images/ab177932-205896-anti-adh5-antibody-epr12885b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)](https://www.abcam.com/ps/products/177/ab177932/Images/ab177932-205898-anti-adh5-antibody-epr12885b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-ADH5 antibody [EPR12885(B)] (ab177932)](https://www.abcam.com/ps/products/177/ab177932/Images/ab177932-9-benefits-of-recombinant-antibodies.png)
Anti-ADH5 antibody [EPR12885(B)] (ab177932)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab177932 has been referenced in 3 publications.
- Chen G et al. bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway. Redox Biol 41:101904 (2021). PubMed: 33706169
- Pan L et al. S-Nitrosylation of Plastin-3 Exacerbates Thoracic Aortic Dissection Formation via Endothelial Barrier Dysfunction. Arterioscler Thromb Vasc Biol 40:175-188 (2020). PubMed: 31694393
- Chen W et al. Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. J Cell Mol Med 23:8161-8172 (2019). PubMed: 31565850