Recombinant
RabMAb

Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283)

Overview

  • Product name
    Anti-ADH5 antibody [EPR12886(B)] - C-terminal
    See all ADH5 primary antibodies
  • Description
    Rabbit monoclonal [EPR12886(B)] to ADH5 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ADH5 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P11766

  • Positive control
    • Human fetal liver, Human fetal kidney, K562 and HepG2 lysates; Human heart and liver tissue; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
    • Sequence similarities
      Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADH 3 antibody
      • ADH5 antibody
      • ADHX antibody
      • ADHX_HUMAN antibody
      • Alcohol dehydrogenase (class III) chi polypeptide antibody
      • alcohol dehydrogenase 5 (class III) chi polypeptide antibody
      • Alcohol dehydrogenase 5 antibody
      • Alcohol dehydrogenase class 3 antibody
      • Alcohol dehydrogenase class chi chain antibody
      • Alcohol dehydrogenase class III antibody
      • Alcohol dehydrogenase class-3 antibody
      • Alcohol dehydrogenase class-III antibody
      • class III alcohol dehydrogenase 5 chi subunit antibody
      • FALDH antibody
      • FDH antibody
      • formaldehyde dehydrogenase antibody
      • Glutathione dependent formaldehyde dehydrogenase antibody
      • Glutathione-dependent formaldehyde dehydrogenase antibody
      • GSH-FDH antibody
      • GSNOR antibody
      • hydroxymethyllutathione dehydrogenase antibody
      • S-(hydroxymethyl)glutathione dehydrogenase antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized K562 cells labeling ADH5 with ab174283 at 1/50 (red)  or a rabbit IgG (negative) (green).

    • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling ADH5 with ab174283 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling ADH5 with ab174283 at 1/50.

    • All lanes : Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : K562 cell line lysate
      Lane 4 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution

      Developed using the ECL technique.

      Predicted band size: 40 kDa

    References

    ab174283 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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