Product nameAnti-ADH5 antibody [EPR12886(B)] - C-terminal
See all ADH5 primary antibodies
DescriptionRabbit monoclonal [EPR12886(B)] to ADH5 - C-terminal
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ADH5 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P11766
- Human fetal liver, Human fetal kidney, K562 and HepG2 lysates; Human heart and liver tissue; K562 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 40 kDa.|
FunctionClass-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
- Information by UniProt
- ADH 3 antibody
- ADH5 antibody
- ADHX antibody
All lanes : Anti-ADH5 antibody [EPR12886(B)] - C-terminal (ab174283) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : K562 cell line lysate
Lane 4 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Flow cytometric analysis of permeabilized K562 cells labeling ADH5 with ab174283 at 1/50 (red) or a rabbit IgG (negative) (green).
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling ADH5 with ab174283 at 1/50.
Immunohistochemical analysis of paraffin embedded Human heart tissue labeling ADH5 with ab174283 at 1/50.
ab174283 has not yet been referenced specifically in any publications.