Recombinant
RabMAb

Recombinant Anti-ADH7 antibody [EPR13949] (ab186408)

Overview

  • Product name

    Anti-ADH7 antibody [EPR13949]
    See all ADH7 primary antibodies
  • Description

    Rabbit monoclonal [EPR13949] to ADH7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Common marmoset
  • Immunogen

    Synthetic peptide within Human ADH7 aa 1-100. The exact sequence is proprietary.
    Database link: P40394

  • Positive control

    • Human stomach and fetal liver lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • Relevance

    This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.
  • Cellular localization

    Cytoplasm
  • Database links

  • Alternative names

    • ADH 4 antibody
    • ADH 7 antibody
    • ADH, class IV antibody
    • ADH4 antibody
    • Alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide antibody
    • alcohol dehydrogenase 7 antibody
    • alcohol dehydrogenase class 4 mu/sigma chain antibody
    • Alcohol dehydrogenase class IV mu/sigma chain antibody
    • alcohol dehydrogenase VII antibody
    • class IV sigma 1 alcohol dehydrogenase antibody
    • class IV sigmasigma alcohol dehydrogenase antibody
    • Gastric alcohol dehydrogenase antibody
    • Gastric H(+)/K(+) ATPase subunit alpha antibody
    • Retinol dehydrogenase antibody
    see all

Images

  • All lanes : Anti-ADH7 antibody [EPR13949] (ab186408) at 1/1000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling ADH7 with ab186408 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

References

ab186408 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Stomach
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Feb 17 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Feb 17 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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