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    adhfe1-antibody-epr12501-ab173530.pdf

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Signal Transduction Metabolism Alcohol metabolism
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RecombinantRabMAb

Recombinant Anti-ADHFE1 antibody [EPR12501] (ab173530)

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  • SDS
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Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] (ab173530)
  • Western blot - Anti-ADHFE1 antibody [EPR12501] (ab173530)
  • Anti-ADHFE1 antibody [EPR12501] (ab173530)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12501] to ADHFE1
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)

View more associated products

Overview

  • Product name

    Anti-ADHFE1 antibody [EPR12501]
    See all ADHFE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12501] to ADHFE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human ADHFE1. The exact sequence is proprietary.
    Database link: Q8IWW8

  • Positive control

    • Human fetal liver and fetal kidney lysates. Immunoprecipitation pellet from Human fetal liver lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12501
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab173530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
IP
1/10 - 1/100.
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Target information above from: UniProt accession Q8IWW8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 137872 Human
    • Omim: 611083 Human
    • SwissProt: Q8IWW8 Human
    • Unigene: 720023 Human
    • Alternative names

      • ADH8 antibody
      • ADHFE 1 antibody
      • ADHFe1 antibody
      • Alcohol dehydrogenase iron-containing protein 1 antibody
      • Alcohol dehydrogenase, iron containing, 1 antibody
      • Fe containing alcohol dehydrogenase antibody
      • Fe-containing alcohol dehydrogenase antibody
      • HOT antibody
      • HOT_HUMAN antibody
      • Hydroxyacid oxoacid transhydrogenase, mitochondrial antibody
      • Hydroxyacid-oxoacid transhydrogenase antibody
      • mitochondrial antibody
      see all

    Images

    • Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] (ab173530)
      Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] (ab173530)

      Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver lysate or (Lane 2) 1X PBS (negative control) using ab173530 at a 1/10 dilution to immunoprecipitate ADHFE1, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Western blot - Anti-ADHFE1 antibody [EPR12501] (ab173530)
      Western blot - Anti-ADHFE1 antibody [EPR12501] (ab173530)
      All lanes : Anti-ADHFE1 antibody [EPR12501] (ab173530) at 1/1000 dilution

      Lane 1 : Human fetal liver lysates
      Lane 2 : Human fetal kidney lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Anti-ADHFE1 antibody [EPR12501] (ab173530)
      Anti-ADHFE1 antibody [EPR12501] (ab173530)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab173530? Please let us know so that we can cite the reference in this datasheet.

    ab173530 has not yet been referenced specifically in any publications.

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