Recombinant Anti-ADHFE1 antibody [EPR12501] (ab173530)
![Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] (ab173530)](https://www.abcam.com/ps/products/173/ab173530/Images/ab173530-197074-anti-adhfe1-antibody-epr12501-immunoprecipitation.jpg "Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] (ab173530)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12501] to ADHFE1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ADHFE1 antibody [EPR12501]
See all ADHFE1 primary antibodies -
Description
Rabbit monoclonal [EPR12501] to ADHFE1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment within Human ADHFE1. The exact sequence is proprietary.
Database link: Q8IWW8 -
Positive control
- Human fetal liver and fetal kidney lysates. Immunoprecipitation pellet from Human fetal liver lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12501 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
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| IP |
1/10 - 1/100.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
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IP
1/10 - 1/100. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 137872 Human
- Omim: 611083 Human
- SwissProt: Q8IWW8 Human
- Unigene: 720023 Human
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Alternative names
- ADH8 antibody
- ADHFE 1 antibody
- ADHFe1 antibody
see all
Images
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Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] (ab173530)
Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver lysate or (Lane 2) 1X PBS (negative control) using ab173530 at a 1/10 dilution to immunoprecipitate ADHFE1, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Western blot - Anti-ADHFE1 antibody [EPR12501] (ab173530)](https://www.abcam.com/ps/products/173/ab173530/Images/ab173530-197072-anti-adhfe1-antibody-epr12501-western-blot.jpg)
Western blot - Anti-ADHFE1 antibody [EPR12501] (ab173530)All lanes : Anti-ADHFE1 antibody [EPR12501] (ab173530) at 1/1000 dilution
Lane 1 : Human fetal liver lysates
Lane 2 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa -
![Anti-ADHFE1 antibody [EPR12501] (ab173530)](https://www.abcam.com/ps/products/173/ab173530/Images/ab173530-3-benefits-of-recombinant-antibodies.png)
Anti-ADHFE1 antibody [EPR12501] (ab173530)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab173530 has not yet been referenced specifically in any publications.