Anti-ADHFE1 antibody [EPR12501] Recombinant (ab173530)

Product name

Anti-ADHFE1 antibody [EPR12501]
See all ADHFE1 primary antibodies
Description

Rabbit monoclonal [EPR12501] to ADHFE1
Host species

Rabbit
Tested applications

Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivity

Reacts with: Human
Does not react with: Mouse, Rat
Immunogen

Recombinant fragment within Human ADHFE1. The exact sequence is proprietary.
Database link: Q8IWW8
Positive control
- Human fetal liver and fetal kidney lysates. Immunoprecipitation pellet from Human fetal liver lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12501
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab173530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
IP		1/10 - 1/100.

Application notes

Is unsuitable for ICC/IF or IHC-P.

Target information above from: UniProt accession Q8IWW8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 137872 Human
- Omim: 611083 Human
- SwissProt: Q8IWW8 Human
- Unigene: 720023 Human
Alternative names
- ADH8 antibody
- ADHFE 1 antibody
- ADHFe1 antibody
- Alcohol dehydrogenase iron-containing protein 1 antibody
- Alcohol dehydrogenase, iron containing, 1 antibody
- Fe containing alcohol dehydrogenase antibody
- Fe-containing alcohol dehydrogenase antibody
- HOT antibody
- HOT_HUMAN antibody
- Hydroxyacid oxoacid transhydrogenase, mitochondrial antibody
- Hydroxyacid-oxoacid transhydrogenase antibody
- mitochondrial antibody
see all

Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] (ab173530)

Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver lysate or (Lane 2) 1X PBS (negative control) using ab173530 at a 1/10 dilution to immunoprecipitate ADHFE1, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Western blot - Anti-ADHFE1 antibody [EPR12501] (ab173530)

All lanes : Anti-ADHFE1 antibody [EPR12501] (ab173530) at 1/1000 dilution

Lane 1 : Human fetal liver lysates
Lane 2 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 50 kDa

ab173530 has not yet been referenced specifically in any publications.

Publishing research using ab173530? Please let us know so that we can cite the reference in this datasheet.

Anti-ADHFE1 antibody [EPR12501] (ab173530)