Key features and details
- Assay type: Cell-based
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells
Product nameAdipogenesis Assay Kit (Cell-Based)
See all Adipogenesis kits
Sample typeAdherent cells
Species reactivityReacts with: Mammals, Other species
Adipogenesis Assay Kit (ab133102) provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model using the adipogenesis induction procedure. This kit can also be used to screen drug candidates involved in adipogenesis. The classic Oil Red O staining for lipid droplets is used in this kit as an indicator of the degree of adipogenesis and can be quantified with a plate reader after the dye is conveniently extracted from the lipid droplet.
Obesity is a risk factor in many of the major chronic diseases such as diabetes mellitus, and cancer. The ability to regulate the number of fat cells and/or the size of fat cells is a key in the development and physiology of obesity and also in the origin of chronic disease. Mammals have both brown adipose tissue (BAT), which utilizes lipids to generate heat in a process known as thermogenesis, and white adipose tissue (WAT) which stores excess energy as triglycerides in lipid droplets. Adipocytes are derived from multipotent mesenchymal precursor cells commit to preadipocytes and then either remain dormant or proceed to become differentiated adipocytes.
3T3-L1 cells are a well-characterized cell line to study the differentiation of adipocytes, and other mechanisms such as insulin-induced glucose uptake and mechanisms of obesity development. This model system has greatly advanced the understanding of the molecular basis and signaling pathways of adipogenesis. During terminal differentiation, the fibroblast-like preadipocytes undergo a series of morphological and biochemical changes to eventually accumulate lipid droplets. This in vitro differentitated adipocytes share similar morphology with adipocytes in vivo.
Storage instructionsPlease refer to protocols.
Components 1 kit Adipogenesis Assay Dexamethasone Solution 1 x 500µl Adipogenesis Assay Insulin Solution (1,000X) 1 x 1.5ml Cell Based Assay IBMX Solution (1,000X) 1 x 500µl Fixative (10X) 1 x 10ml Lipid Droplets Assay Dye Extraction Solution 1 x 30ml Lipid Droplets Assay Oil Red O Solution 1 x 25ml Lipid Droplets Assay Wash Solution 6 x 30ml
RelevanceAdipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
Lipid droplet accumulation in the differentiated 3T3-L1 cells visualized by Oil Red O Solution staining.
Upon induction, 3T3-L1 cells differentiate into adipocytes-like cells and they are ready to be used for adipogenesis experiments.
Panel A: Non-differentiated 3T3-L1 cells were not stained by Oil Red O Solution.
Panel B: more than 80% of preadipocytes were differentiated four days after weaning the cells from induction medium to insulin medium. Lipid droplet accumulation in the differentiated cells can be visualized by Oil Red O Solution staining.
ab133102 has been referenced in 5 publications.
- Ida Y et al. Simultaneous Use of ROCK Inhibitors and EP2 Agonists Induces Unexpected Effects on Adipogenesis and the Physical Properties of 3T3-L1 Preadipocytes. Int J Mol Sci 22:N/A (2021). PubMed: 33925005
- Ida Y et al. Omidenepag, a non-prostanoid EP2 receptor agonist, induces enlargement of the 3D organoid of 3T3-L1 cells. Sci Rep 10:16018 (2020). PubMed: 32994409
- Torres-Villarreal D et al. Anti-obesity effects of kaempferol by inhibiting adipogenesis and increasing lipolysis in 3T3-L1 cells. J Physiol Biochem 75:83-88 (2019). PubMed: 30539499
- Camacho A et al. Tyrphostin AG17 inhibits adipocyte differentiation in vivo and in vitro. Lipids Health Dis 17:128 (2018). PubMed: 29843731
- Li Y et al. Suppression of adipocyte differentiation and lipid accumulation by stearidonic acid (SDA) in 3T3-L1 cells. Lipids Health Dis 16:181 (2017). Functional Studies ; Mouse . PubMed: 28946872