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  1. Link

    adiponectin-antibody-19f1-ab22554.pdf

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Cardiovascular Lipids / Lipoproteins Adipose Related Acrp
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Anti-Adiponectin antibody [19F1] (ab22554)

  • Datasheet
  • SDS
Reviews (16)Q&A (9)References (81)

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Western blot - Anti-Adiponectin antibody [19F1] (ab22554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
  • Immunocytochemistry - Anti-Adiponectin antibody [19F1] (ab22554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
  • Immunocytochemistry - Anti-Adiponectin antibody [19F1] (ab22554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)

Key features and details

  • Mouse monoclonal [19F1] to Adiponectin
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Adiponectin antibody [19F1]
    See all Adiponectin primary antibodies
  • Description

    Mouse monoclonal [19F1] to Adiponectin
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control

    • 3T3-L1 adipocytes
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    19F1
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Acrp
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Neurology process
    • Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Recombinant Protein

    • Recombinant Human Adiponectin protein (ab51294)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab22554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use a concentration of 2 µg/ml.
IHC-P (5)
Use at an assay dependent concentration.
WB (10)
Use a concentration of 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
Notes
ICC
Use a concentration of 2 µg/ml.
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).

Target

  • Function

    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificity

    Synthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in disease

    Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Domain

    The C1q domain is commonly called the globular domain.
  • Post-translational
    modifications

    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q15848 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9370 Human
    • Entrez Gene: 11450 Mouse
    • Omim: 605441 Human
    • SwissProt: Q15848 Human
    • SwissProt: Q60994 Mouse
    • Unigene: 80485 Human
    • Unigene: 3969 Mouse
    • Alternative names

      • 30 kDa adipocyte complement related protein antibody
      • 30 kDa adipocyte complement-related protein antibody
      • ACDC antibody
      • Acrp 30 antibody
      • ACRP30 antibody
      • ADIPO_HUMAN antibody
      • Adipocyte antibody
      • Adipocyte C1q and collagen domain containing protein antibody
      • Adipocyte complement related 30 kDa protein antibody
      • Adipocyte complement related protein of 30 kDa antibody
      • Adipocyte complement-related 30 kDa protein antibody
      • adipocyte-specific secretory protein antibody
      • Adiponectin antibody
      • Adiponectin precursor antibody
      • adiponectin, C1Q and collagen domain containing antibody
      • Adipoq antibody
      • Adipose most abundant gene transcript 1 antibody
      • Adipose most abundant gene transcript 1 protein antibody
      • Adipose specific collagen like factor antibody
      • ADIPQTL1 antibody
      • ADPN antibody
      • APM 1 antibody
      • apM-1 antibody
      • APM1 antibody
      • C1q and collagen domain-containing protein antibody
      • GBP 28 antibody
      • GBP28 antibody
      • Gelatin binding protein antibody
      • Gelatin binding protein 28 antibody
      • gelatin-binding protein 28 antibody
      • Gelatin-binding protein antibody
      • OTTHUMP00000210047 antibody
      see all

    Images

    • Western blot - Anti-Adiponectin antibody [19F1] (ab22554)
      Western blot - Anti-Adiponectin antibody [19F1] (ab22554)
      Anti-Adiponectin antibody [19F1] (ab22554) at 2 µg/ml + Hep G2 cell line

      Secondary
      Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP at 1/2500 dilution

      Predicted band size: 26 kDa



      A ~26 kDa band corresponding Adiponectin was observed in the cell line tested.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse Adipose tissue staining Adiponectin using ab22554 (PBS in negative control) at 1:200 (5μg/ml). ImmunoHistoProbe one step HRP Polymer (ready to use) used. Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Immunocytochemistry - Anti-Adiponectin antibody [19F1] (ab22554)
      Immunocytochemistry - Anti-Adiponectin antibody [19F1] (ab22554)

      ab22554 staining Adiponectin in 3T3-L1 cells (ATCC® CL-173 TM). Increased expression of Adiponectin correlates with adipocyte phenotype, as described in literature. Cells, grown to confluency in DMEM with 10% FBS, were differentiated by stimulation for two days with 0.5 mM 3-isobutyl-1-methylxanthi​ne (ab120840), 0.25uM dexamethasone (ab120743) and 1ug/ml insulin (ab123768), followed by two more days with only insulin. Cells were maintained for an additional three days in growth medium alone. Undifferentiated and differentiated adipocytes were fixed with 100% methanol (5min) at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature. Staining of the treated cells with ab22554 (2.5µg/ml) and ab6046 at 1µg/ml overnight at +4°C, was followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 2 µg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (ab150120) at 2 µg/ml (shown in pseudo colour red). Nuclear DNA was labelled in blue with DAPI. Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
      Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Adiponectin ab22554 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunocytochemistry - Anti-Adiponectin antibody [19F1] (ab22554)
      Immunocytochemistry - Anti-Adiponectin antibody [19F1] (ab22554)Image courtesy of an anonymous Abreview.
      ab22554 staining Adiponectin in human adipose stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X and then blocked using 4% serum for 1 hour. Samples were then incubated with primary antibody at 1/500 for 1 hour 30 minutes. The secondary antibody used was a goat IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)This image is courtesy of an anonymous Abreview
      ab22554 staining Adiponectin in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
      ab22554 (4µg/ml) staining adiponectin in human breast, using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and cytoplasmic staining.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody [19F1] (ab22554)
      Immunohistochemistry was performed on biopsies of deparaffinized Human skin tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Adiponectin ab22554 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (81)

    Publishing research using ab22554? Please let us know so that we can cite the reference in this datasheet.

    ab22554 has been referenced in 81 publications.

    • Luo P  et al. S-Allylmercaptocysteine improves alcoholic liver disease partly through a direct modulation of insulin receptor signaling. Acta Pharm Sin B 11:668-679 (2021). PubMed: 33777674
    • Chen L  et al. Injectable bio-responsive hydrogel for therapy of inflammation related eyelid diseases. Bioact Mater 6:3062-3073 (2021). PubMed: 33778188
    • Liu J  et al. Exosomes from tamoxifen-resistant breast cancer cells transmit drug resistance partly by delivering miR-9-5p. Cancer Cell Int 21:55 (2021). PubMed: 33451320
    • Wagle S  et al. Supplemental Ferulic Acid Inhibits Total Body Irradiation-Mediated Bone Marrow Damage, Bone Mass Loss, Stem Cell Senescence, and Hematopoietic Defect in Mice by Enhancing Antioxidant Defense Systems. Antioxidants (Basel) 10:N/A (2021). PubMed: 34439457
    • Chen PY  et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms. Cell Stem Cell 26:542-557.e11 (2020). PubMed: 32243809
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 25 Abreviews or Q&A

    Western blot abreview for Anti-Adiponectin antibody [19F1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mammary epithelial cells)
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Loading amount
    25 µg
    Specification
    mammary epithelial cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Flavia Ghiraldini

    Verified customer

    Submitted Oct 08 2018

    Western blot abreview for Anti-Adiponectin antibody [19F1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (adipose tissue)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    50 µg
    Specification
    adipose tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 30°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 09 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Adiponectin antibody [19F1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (vessel)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    vessel
    Blocking step
    dako antibody diluent with background reducing components as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Miss. Seulgee Lee

    Verified customer

    Submitted Feb 21 2017

    Western blot abreview for Anti-Adiponectin antibody [19F1]

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Serum (serum (I used 10ul of 1:100 dilution of serum))
    Gel Running Conditions
    Reduced Denaturing (4-20% tris glycine)
    Loading amount
    1 µg
    Specification
    serum (I used 10ul of 1:100 dilution of serum)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 07 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Adiponectin antibody [19F1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: High pH
    Sample
    Human Tissue sections (Tendon)
    Specification
    Tendon
    Permeabilization
    No
    Fixative
    10% buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 22 2014

    Western blot abreview for Anti-Adiponectin antibody [19F1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 23 2014

    Western blot abreview for Anti-Adiponectin [19F1] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    12 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Heart)
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 22 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Adiponectin antibody [19F1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (fat)
    Specification
    fat
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 06 2013

    Western blot abreview for Anti-Adiponectin antibody [19F1]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Epi fat & subcutaneous fat)
    Loading amount
    30 µg
    Specification
    Epi fat & subcutaneous fat
    Gel Running Conditions
    Reduced Denaturing (10% SDS)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 31 2012

    Question

    Thank you very much for your prompt reply!

    The antibody that I have seems to be working (or atleast good at giving a specific band) however, I am not sure why it is at a size (50-60 kDa) that is different that what Adiponectin would ideally come at. Have you come across any other users of this antibody detecting a specific band in this size range (50-60kDa)?

    I do not mind trying a new antibody as long as it works but I just wanted to make sure about this issue that I am having. HepG2 cells are not commonly studied for adiponectin expression. Moreover, I read another review under this antibody where the used claims a band at 50 kDa. Is that common?

    Thanks in advance!!

    Read More

    Abcam community

    Verified customer

    Asked on Jun 26 2012

    Answer

    Thank you for your reply.

    It is actually common to see dimers and multimers of adiponectin, so this is probably the case with your experiment. Please see the references below:

    http://www.jbc.org/content/278/41/40352.full.pdf+html

    I hope this information helps. If you're still not satisfied with the results you received, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Jun 26 2012

    1-10 of 25 Abreviews or Q&A

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